Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • 3,3′-dihexyloxacarbocyanine iodide  (1)
  • Amyloplast  (1)
  • DNA-containing organelle  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Unequal distribution of DNA-containing organelles in generative and sperm cells of Erythrina crista-galli (Fabaceae) (2000)
    Springer
    Sexual plant reproduction 12 (2000), S. 296-301 
    Details
    ISSN: 1432-2145
    Keywords: Key words Erythrina ; Generative cell ; DNA-containing organelle ; Plumbago ; Preferential double fertilization ; Sperm cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The generative cell at anthesis in the mature pollen grain of Erythrina crista-galli (Fabaceae) was examined by 4,6-diamidino-2-phenylindole(DAPI)-fluorescence microscopy using the squash method. An unequal, polarized distribution of DNA-containing organelles (plastids and/or mitochondria) within the generative cell was observed in every mature pollen grain examined. Polarization of DNA-containing organelles is obvious when generative cells are freed and assume a spherical shape soon after microspore mitosis, as revealed by fluorescence-microscopic observations of specimens embedded in Technovit 7100 resin and thin-sectioned at different developmental stages. Early establishment of polarized localization of organelles in young generative cells of E. crista-galli and maintenance of this unequal distribution until pollen maturation strongly suggests that the organelles may still be clustered at pollen mitosis. Production of a dimorphic pair of sperm cells, as has been reported in Plumbago zeylanica, was observed in some pollen tubes germinated in vitro. The differentiation of the two sperm cells is discussed in relation to possible preferential double fertilization in angiosperms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050198
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Amyloplast formation in cultured tobacco cells. III Determination of the timing of gene expression necessary for starch accumulation (1999)
    Springer
    Plant cell reports 18 (1999), S. 589-594 
    Details
    ISSN: 1432-203X
    Keywords: Key words ADP-glucose starch glycosyl transferase ; Amyloplast ; BY-2 ; Nicotiana tabacum ; Transcription/translation inhibitors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When BY-2 cultured tobacco (Nicotiana tabacum L.) cells were transferred to auxin-depleted culture medium containing cytokinin (benzyladenine, 1 mg/l), the starch content per cell started increasing from 18 h of culture and amyloplasts had formed by 48 h. Pulse-treatment of the cells with actinomycin D and cycloheximide for the first 12 h (or longer) of culture significantly decreased the cellular starch content after 48 h, whereas the starch content did not decrease significantly when the cells were released from the inhibition within 6 h. This suggests that nuclear gene expression necessary for amyloplast formation begins 6–12 h after the transfer. Immunoblotting analysis of the accumulation of ADP-glucose starch glycosyl transferase (starch synthase) supported this inference.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050627
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Unique positioning of mitochondria in developing microspores and pollen grains inPharbitis nil: mitochondria cover the nuclear surface at specific developmental stages (2000)
    Springer
    Protoplasma 213 (2000), S. 74-82 
    Details
    ISSN: 1615-6102
    Keywords: 3,3′-dihexyloxacarbocyanine iodide ; Male gametogenesis ; Mitochondria ; Nuclear envelope ; Pollen ; Pharbitis nil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Changes in the number and distribution of mitochondria in microspores and pollen grains during male gametogenesis inPharbitis nil were examined with Technovit sections stained with 3,3′-dihexyloxacarbocyanine iodide. The number of mitochondria per microspore or pollen grain ofP. nil increased constantly and dramatically during male gametogenesis. During this process, mitochondria exhibited characteristic localizations: subpopulations of mitochondria covered the surface of the microspore and vegetative nuclei before and again just after postmeiotic mitosis I (9 and 5 days before flowering, respectively). The mitochondria also surrounded the generative nucleus 2 days after postmeiotic mitosis I (5 days before flowering), although the density of mitochondria on the nuclear surface was lower. Electron microscopy showed that the mitochondria were about 30 nm from the nuclear envelope and that each mitochondrion was located near a nuclear pore. The characteristic localization of mitochondria inP. nil pollen may serve as a model to analyze the mechanisms that control mitochondrial positioning within a cell and interactions between mitochondria and nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01280507
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...