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  • 3β-HSD  (1)
  • Dehydroepiandrosterone  (1)
  • Human skin  (1)
  • Monoclonal antibody  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Monoclonal antibodies to the β-andrenergic receptor: Modulation of catecholamine-sensitive adenylate cyclase by the antibody (1986)
    Springer
    Archives of dermatological research 278 (1986), S. 377-381 
    Details
    ISSN: 1432-069X
    Keywords: β-Adrenergic receptor ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We have developed two types of hybridomas producing monoclonal antibodies to the turkey erythrocyte β1-adrenergic receptor in order to study the β-adrenergic-cAMP system of epidermis. Splenic cells from BALB/c mice immunized with partially purified turkey erythrocyte β1-adrenergic were fused with mouse myeloma cell line SP2/0-Ag14. Five hybridomas of 17 positive cells producing antibodies which could precipitate soluble turkey erythrocyte β1-receptors were cloned by the limiting dilution method. The antibodies cross-reacted with β- and β2-adrenergic receptors and stained epidermal basal cells with immunocytochemical techniques. Neither type of antibody interfered with the antagonist binding, i.e., all antibodies bound to sites other than the ligand binding site on the surface. One type of antibody inhibited epinephrine-stimulated adenylate cyclase activity in our “leaky” epidermal cell system. The data suggest that the antibody interferes with the coupling of the receptor to the regulatory protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00418166
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Activity of 3β-hydroxysteroid dehydrogenase Δ4–5 isomerase in the human skin (1982)
    Springer
    Archives of dermatological research 274 (1982), S. 289-294 
    Details
    ISSN: 1432-069X
    Keywords: 3β-HSD ; Human skin ; Sebaceous gland ; Dehydroepiandrosterone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The activity of 3β-hydroxysteroid dehydrogenase Δ4–5 isomerase (3β-HSD) was assayed in various tissues microdissected from the freeze-dried human skin of 13 subjects. The sebaceous gland possessed the highest activity of 3β-HSD in the skin, while the apocrine sweat gland showed only one fourth of that activity. The vellus hair follicle showed nearly one half of the activity of the sebaceous gland, whereas the terminal hair follicle exhibited much lower activity. The activity of the epidermis and of the dermis were negligible. Incubation of fresh whole skin of the forehead with 7α-3H-DHA and subsequent isolation of various tissues revealed that the metabolites identified in the sebaceous gland were androstanedione and androstenedione, whereas testosterone and/or dihydrotestosterone were not detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00403732
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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