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  • 1
    Electronic Resource
    Electronic Resource
    A novel carotenoid biosynthesis gene coding for ζ-carotene desaturase: functional expression, sequence and phylogenetic origin (1994)
    Springer
    Plant molecular biology 24 (1994), S. 369-379 
    Details
    ISSN: 1573-5028
    Keywords: Anabaena PCC 7120 ; carotenoid biosynthesis ; ζ-carotene desaturase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A DNA fragment which has been isolated previously from an Anabaena DNA expression library was subcloned. The corresponding protein was overexpressed in Escherichia coli. The recombinant enzyme was fully active in converting ζ-carotene into lycopene in vitro with neurosporene as an intermediate. A smaller fragment which still contained the active enzyme was sequenced. An open reading frame of 1497 bp was found coding for a protein consisting of 499 amino acids with the calculated molecular weight of 56 740. In a computer search of nucleotide sequences contained in the EMBL nucleotide sequence library, all the best-fitting comparisons were carotenoid desaturases. The highest similarity was found with the crtI phytoene desaturase genes of bacteria and the al-1 gene from Neurospora crassa. A much lower similarity was found with the pds genes coding for phytoene desaturase from cyanobacteria and higher plants. It is shown in protein similarity plots that the amino acid similarity of ζ-carotene desaturase to the latter is mainly limited to the N terminus of the polypeptides. In contrast, the protein similarity plots and a comparison of a conserved region clearly demonstrate that there is a strong relationship between ζ-carotene desaturase and the phytoene desaturases from various bacteria and fungi. Therefore we propose that the ζ-carotene desaturase gene is homologous to the crt I phytoene desaturase genes of bacteria and fungi.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020174
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of light on plastocyanin formation in the alga Scenedesmus acutus (1981)
    Springer
    Photosynthesis research 2 (1981), S. 281-289 
    Details
    ISSN: 1573-5079
    Keywords: ATP level ; autotrophic/heterotrophic cells ; plastocyanin formation ; role of light ; 35S-sulfolipid ; thylakoid formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Scenedesmus acutus, pregrown autotrophically, forms high levels of plastocyanin during an initiation period of 24 hr in the light, after cupric ions have been added to depleted cells. In the dark, no plastocyanin formation is observed. This is in contrast to heterotrophic cells which, over the same period and under identical conditions, yield about half the plastocyanin level regardless of whether they are incubated in the dark or in the light. The ATP level is high in all cases when substantial formation of plastocyanin takes place, while the 35S-sulfolipid level, the marker of thylakoid formation, is at variance. After the 24-hr plastocyanin formation period, the 35S-sulfolipid level is high in illuminated autotrophic cells as well as in dark-incubated heterotrophic ones, but low in illuminated heterotrophic cells, all of them having substantial plastocyanin contents. Additional experiments on light dependence of copper uptake, influence of light quality, occurrence of apoplastocyanins, provide a set of data to assume that the role of light in plastocyanin formation is primarily through photophosphorylation, the ATP presumably being necessary for processing of plastocyanin precursors to holoprotein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00056265
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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