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  • 1
    Electronic Resource
    Electronic Resource
    Experimental study of enhanced emission of the laser-ablated plume in backing gas (1994)
    Springer
    Applied physics 58 (1994), S. 143-148 
    Details
    ISSN: 1432-0649
    Keywords: 36.40
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The effects of background gases on the optical emission of the excimer-laser-ablated plume from a brass target have been studied experimentally. It is found that the plume emission can be enhanced significantly in a proper gas ambient. In hydrogen, the highest peak intensity is detected, and in argon, there is a distinctive difference in the pressure-dependent emission between in He and in the other three gases, Ar, N2 and H2. Moreover, the monitored line peak intensity remains unchanged in Ar and N2 and increases in H2 within a distance above the target surface; but in He, the observed peak intensity decreases with distance like in vacuum. Furthermore, the emissions of several more atomic lines of Cu and Zn atoms from the plume are also found to be enhanced in the same manner in gas ambient. Some physical processes involved in the plume expansion and the possible mechanisms for the enhanced emission of the plume in backing gas are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01082349
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells (1997)
    Springer
    Plant cell reports 16 (1997), S. 379-384 
    Details
    ISSN: 1432-203X
    Keywords: Forage and turf grasses ; Italian ryegrassLolium multiflorum ; Microprojectile bombardment ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic forage-type Italian ryegrass (Lolium multiflorum Lam.) plants have been obtained by microprojectile bombardment of embryogenic suspension cells using a chimeric hygromycin phosphotransferase (hph) gene construct driven by riceActl 5′ regulatory sequences. Parameters for the bombardment of embryogenic suspension cultures with the particle inflow gun were partially optimized using transient expression assays of a chimericβ-glucuronidase (gusA) gene driven by the maizeUbi1 promoter. Stably transformed clones were recovered with a selection scheme using hygromycin in liquid medium followed by a plate selection. Plants were regenerated from 33% of the hygromycin-resistant calli. The transgenic nature of the regenerated plants was demonstrated by Southern hybridization analysis. Expression of the transgene in transformed adult Italian ryegrass plants was confirmed by northern analysis and a hygromycin phosphotransferase enzyme assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146777
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Transgenic Italian ryegrass (Lolium multiflorum) plants from microprojectile bombardment of embryogenic suspension cells (1997)
    Springer
    Plant cell reports 16 (1997), S. 379-384 
    Details
    ISSN: 1432-203X
    Keywords: Key words Forage and turf grasses ; Italian ryegrass ; Lolium multiflorum ; Microprojectile bombardment ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic forage-type Italian ryegrass (Lolium multiflorum Lam.) plants have been obtained by microprojectile bombardment of embryogenic suspension cells using a chimeric hygromycin phosphotransferase (hph) gene construct driven by rice Act1 5′ regulatory sequences. Parameters for the bombardment of embryogenic suspension cultures with the particle inflow gun were partially optimized using transient expression assays of a chimeric β-glucuronidase (gusA) gene driven by the maize Ubi1 promoter. Stably transformed clones were recovered with a selection scheme using hygromycin in liquid medium followed by a plate selection. Plants were regenerated from 33% of the hygromycin-resistant calli. The transgenic nature of the regenerated plants was demonstrated by Southern hybridization analysis. Expression of the transgene in transformed adult Italian ryegrass plants was confirmed by northern analysis and a hygromycin phosphotransferase enzyme assay.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01146777
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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