ISSN:
1573-4935
Keywords:
Biotin-GM1
;
immuno-electron microscopy
;
ganglioside transport
;
endocytosis
;
intralysosomal membranes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract A biotin-labeled derivative of the ganglioside GM1 (biotin-GM1) was used to study its transport along the endocytic pathway of cultured fibroblasts by immuno-electron microscopy. Using electron dense endocytic tracers we could demonstrate that late endosomes and lysosomes of these cells are long living organelles with a high content of internal membranes. Our studies show that during endocytosis the biotin-GM1 was transported to these intraendosomal and intralysosomal membranes. These observations support the hypothesis that glycosphingolipids (GSL) are preferentially degraded in intralysosomal vesicles.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1020502525572
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