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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 48 (1989), S. 25-29 
    ISSN: 1432-0649
    Keywords: 42.60Kg ; 35.80+s
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Experimental techniques have been developed to monitor transient infrared absorptions using lead-salt tunable diode lasers. The techniques are easily implemented, yield sensitivities which are limited by detector noise at 10−5 level of absorbance, and have a response time on the order of one microsecond. The transient absorption detection techniques are high frequency versions of the sweep integration technique pioneered by Jennings [Appl. Opt.19, 2695 (1980)]. TDL modulation rates of 100 kHz and 500 kHz allow for absorption sampling rates of 200 kHz and 1 MHz, respectively. In order to reproducibly achieve near-detector-noise-limited sensitivities for 100 kHz TDL modulation rates, an automated analog subtraction circuit has been developed which removes the effects of minor TDL power variations. At the 500 kHz modulation rate, digital filtering techniques are used to remove the effects of this power variation.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Experimental brain research 1 (1966), S. 205-219 
    ISSN: 1432-1106
    Keywords: D-C Potentials ; Hypothalamus ; Cortex ; Midbrain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary D-C potential changes were evoked in the sensorimotor cortex and the hypothalamus of unanesthetized cats with chronically implanted electrodes by repetitive stimulation of the mesencephalic reticular formation. Stimulation at 30 to 300 p/sec. frequencies induced negative d-c shifts which were quite abrupt in onset, starting after the second or third shock with a peak of negativity reached within the first 50 to 100 msec of the 1 second duration of stimulation. Typically these negative shifts were sustained for the duration of stimulation with a prolonged 1 to 1.5 second return to baseline after termination of stimulation. Such d-c shifts were relatively independent of the site of the reference electrode but highly dependent on the location of the “active” recording electrodes and the stimulating electrodes. Reasons have been advanced to suggest that these negative d-c shifts reflect summated neuronal discharge in the immediate environs of “active” recording electrodes in the cerebral cortex and hypothalamus. In contrast to the consistent, stimulus-locked negative d-c shifts, less consistent changes, usually positive in polarity, were observed to begin 2 to 3 seconds after termination of RF stimulation. These slower d-c changes were highly dependent on the site of the reference electrode and the physical state of tissue at the electrode tip and therefore far less directly related to neuronal events in cortical and hypothalamic tissue.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0878
    Keywords: Isotocinergic neurons ; Immunocytochemistry ; Hypothalamus ; Intracellular recording ; Goldfish (Carassius)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isotocinergic (IT) neurons show physiological and morphological characteristics that are similar to those of other preoptic neuroendocrine cells in the goldfish. Preoptic IT cells show resting membrane potentials of 20–55 mV, action potentials of up to 100mV, and physiological evidence of axonal branching. Dye-marked IT cells measure 14–56 μm, their dendrites projecting to the ependyma and into the hypothalamic neuropil, their multiple beaded axons projecting to the pituitary. Indirect immunofluorescence identifies these dyemarked cells as IT. By combining electrophysiological, dye-marking and immunocytochemical techniques we can now, for the first time, study single, antidromically-identified peptidergic neurons of a specific type in vertebrate and invertebrate species.
    Type of Medium: Electronic Resource
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