ISSN:
1572-994X
Keywords:
Epstein-Barr virus interaction
;
DNA polymerase
;
aphidicolin
;
phosphonoformic acid
;
5′-GMP
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Epstein-Barr virus (EBV)-specified DNA polymerase was purified from P3HR-1 cells, a Burkitt lymphoma EBV producer cell line, treated with phorbol-12,13-dibutyrate (PDB) and n-butyrate. Its inhibition by aphidicolin, phosphonoformate (PFA) and 5′-GMP was examined. Aphidicolin could inhibit EBV DNA polymerase competitively with respect to dATP and dCTP and noncompetitively with respect to dGTP and dTTP; whereas 5′-GMP was a noncompetititive inhibitor with respect to all four dNTPs. Combinations of aphidicolin and PFA, or PFA and 5′-GMP, produced a mutually exclusive inhibition pattern of EBV DNA polymerase that suggested that the binding sites of these compounds on the enzyme molcule are kinetically overlapping.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00257099
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