ISSN:
1437-7772
Keywords:
Key words Cancer
;
Indomethacin
;
5-Fluorouracil (5FUra)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Background. The antitumor effect of indomethacin has been well documented in cancer patients and in animals with transplanted tumors. Indomethacin has also been regarded as a biological response modifier. Therefore, we presumed that indomethacin could be a modulator of antitumor agents, and this study was done to determine whether indomethacin modified the cytotoxicity of selected antitumor agents. Methods. The effect of indomethacin on the antitumor activity of 5-fluorouracil (5FUra), cis-platinum (CDDP), and etoposide in Colon 26 tumor cells was evaluated in vitro by MTT assay. Results. Indomethacin did not show a cytotoxic effect on the tumor cells at pharmacological concentrations. Indomethacin preferentially modulated the cytotoxicity of 5FUra, while it did not affect the cytotoxicity of CDDP and etoposide. Several fold indomethacin (0.01 and 0.1 μg/vol) enhanced 5FUra cytotoxicity in tumor cells. Indomethacin increased the 5FUra content in the tumor cells by 80% over the control through increasing the inflow of 5FUra, rather than by prohibiting the efflux of 5FUra. Conclusion. Indomethacin appears to be a candidate for augmenting 5FUra cytotoxicity in tumor cells by facilitating the intracellular uptake of 5FUra. This study suggests the effectiveness of indomethacin in reducing the dosage of 5FUra or in augmenting 5FUra cytotoxicity when given at the same dose.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s101470050018
Permalink
