ISSN:
1432-0428
Keywords:
Isolated fat cells
;
insulin
;
trypsin treatment
;
plasma membranes
;
amino acid incorporation
;
amino acid mixture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Incorporation of 0.1 mM3H-leucine into protein by isolated fat cells, with pyruvate as the only extracellular carbohydrate, was stimulated by: a) insulin, 0.1–4 ng/ml, or b) a mixture of 19 amino acids, 0.05–5 mM. Treatment of fat cells with trypsin, under conditions known to reduce binding of insulin by their plasma membranes, abolished stimulation of incorporation by the hormone but not by the amino acid mixture. These results imply that the actions of insulin on incorporation of amino acids into protein are initiated by interaction with plasma membranes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01239439
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