ISSN:
1432-2013
Schlagwort(e):
Ca2+
;
Smooth muscle
;
Fura-2
;
5-Hydroxytryptamine
;
Forskolin
;
Cyclic AMP
;
Invertebrate
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Abstract Effects of 5-hydroxytryptamine (5-HT) and forskolin on intracellular free calcium concentration ([Ca2+]i) were studied in suspensions of fura-2 loaded smooth-muscle cells from the anterior byssus retractor ‘catch’ muscle ofMytilus edulis. The successive addition of 5 mM carbachol (CCh) and 100 mM KCl to the suspension evoked a transient elevation of [Ca2+]i from the resting value of 124±2.7 nM (mean ± SE,n=18) to 300–400 nM, which was associated with contraction. The change in [Ca2+]i induced CCh was concentration-dependent with the EC50 of 10−5 M. The resting [Ca2+]i was unaffected by 10 μM 5-HT. The change in [Ca2+]i induced by 5 mM CCh was suppressed by 5-HT from 167±14.0 (n=11) to 124±14.9 (n=8) nM whereas that induced by 100 mM KCl was enhanced from 321±31.9 to 405±17.6 nM (n=8). 5-HT applied during the decaying phase of the CCh response caused a rapid decline in [Ca2+]i. In both the responses to CCh and KCl, the falling phase was accelerated by 5-HT. 10 μM forskolin, a potent activator of adenylate cyclase, mimicked the effects of 5-HT as did a membrane-permeant cyclic AMP analogue, 8-parachlorophenylthio cyclic AMP (cpt-cAMP). Application of 100 μM cpt-cAMP partially suppressed the Ca2+ i response to CCh and enhanced that to KCl.d-Tubocurarine (500 μM) added during the decaying phase of the response induced by 100 μM CCh, caused a rapid decline in [Ca2+]i similar to that caused by both 5-HT and forskolin. In essentially Ca2+-free sea water, or in the presence of 10 μM D600 in seawater containing 4 mM, Ca2+, the response to CCh was partially suppressed, whereas that to KCl was completely abolished, demonstrating a CCh-induced release of intracellularly stored Ca2+. The remaining component of the response to CCh, in either Ca2+-free sea water or in the presence of D600, was abolished by both 5-HT and forskolin. The results suggest that 5-HT has multiple effects on [Ca2+]i in the ABRM, and implicate cyclic AMP in this effect, and that one of the mechanisms underlying these responses is the inhibition of an agonist-induced release of stored Ca2+. In addition, that Ca2+ i is at, or close to resting values during the ‘catch state’.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00580959
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