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  • Allium cepa  (2)
  • Type 2 diabetes mellitus  (2)
  • 6-benzyladenine  (1)
  • Cucumis melo  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Protoplast responses in the epidermis of Allium cepa induced by penetration by Botrytis allii (1988)
    Springer
    Mycopathologia 102 (1988), S. 169-173 
    Details
    ISSN: 1573-0832
    Keywords: Allium cepa ; Botrytis allii ; penetration responses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Penetration of Allium cepa epidermal cells (white, yellow, and red varieties) by Botrytis allii induced a response by host protoplasts in normal tissue which was not observed when penetrations were made in protoplast-free host cell walls. Callose and auto-fluorescing substances (possibly phenolic compounds) were located at the penetration sites only in normal host cells containing protoplasts. Lignin tests were negative. Halos were clearly visible in both types of tissue. Autofluorescence was observed at penetration sites in normal cells of all cultivars but general wall background autofluorescence was not observed in white onions. Autofluorescence was generally yellow green and when treated with ammonium hydroxide became green. Treatment with sodium hydroxide abolished autofluorescence. No attempt was made to isolate the autofluorescing material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00437400
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  • 2
    Electronic Resource
    Electronic Resource
    Degenerative changes in the epidermal cell wall of Allium cepa caused by Botrytis allii: Loss of dry mass, pectin, and cellulose in halos (1988)
    Springer
    Mycopathologia 101 (1988), S. 47-52 
    Details
    ISSN: 1573-0832
    Keywords: Botrytis allii ; Allium cepa ; halo composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Halos detected using interference microscopy (even- and fringle-field modes with monoand poly-chromatic light) around penetration sites of Botrytis allii in cell walls of normal and protoplast-free outer epidermal tissue of white, yellow, and red onions were alike. Halos in protoplast-free cell walls contained 33% less dry mass than areas of these walls adjacent to halos (quantitative interference miscroscopy with 546 nm light in the even-field mode). Halos were significantly larger in the white onion than in the yellow and red varieties. The loss of cell wall dry mass during the production of halos involved the loss of pectin and cellulose. We infer that this is caused by enzymes released from the pathogen. Cuticle degradation at penetration sites was not observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00455668
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Circulating endothelin-1 levels in type 2 diabetic patients with ischaemic heart disease (1996)
    Springer
    Acta diabetologica 33 (1996), S. 246-248 
    Details
    ISSN: 1432-5233
    Keywords: Key words Endothelin-1 (Et-1) ; Type 2 diabetes mellitus ; Coronary artery disease (CAD)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate whether circulating endothelin-1 (Et-1) may be related to the increased incidence and severity of ischaemic heart disease in type 2 diabetes mellitus, we compared the concentrations in type 2 diabetic patients and in non-diabetic patients with coronary artery disease (CAD) angiographically documented. Plasma levels of Et-1 were determined in 34 type 2 diabetic patients with CAD (16 with stable angina, 6 with unstable angina, 12 with previous myocardial infarction) and in 19 non-diabetic patients with CAD (4 with stable angina, 5 with unstable angina, 10 with previous myocardial infarction). Fifteen diabetic patients without CAD and 9 healthy volunteers served as control subjects. In the type 2 diabetic patients, the mean Et-1 levels were 3.19±1.61 pmol/l in those with stable angina, 3.58±1.92 pmol/l in those with unstable angina, 4.24±2.53 pmol/l in those with myocardial infarction. These values were not significantly different one another, nor from the values obtained from type 2 diabetic controls (3.64±2.13 pmol/l). In the non-diabetic patients, the mean Et-1 levels were 3.92±0.73 pmol/l in those with stable angina, 4.35±1.67 pmol/l in those with unstable angina, 4.33±1.66 pmol/l in those with myocardial infarction. These values were not significantly different one another, but significantly higher than those obtained from healthy controls (2.07±0.67 pmol/l; P〈0.001). No significant differences were found in Et-1 levels between diabetic and non-diabetic patients with stable, unstable angina and previous myocardial infarction. In contrast, a statistically significant difference was found in Et-1 levels between diabetic and non-diabetic control subjects (P〈0.05). In conclusion, similar raised concentrations of Et-1 in diabetic and non-diabetic patients with stable, unstable angina and previous myocardial infarction do not support the hypothesis that higher levels of Et-1 in diabetic patients are responsible for the increased incidence of CAD in diabetes mellitus. However, the raised Et-1 levels found in diabetic patients in the absence of CAD strongly suggest that a generalised endothelial dysfunction, documented in our study by increased levels of Et-1, most probably precedes subsequent cardiovascular diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02048552
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Circulating endothelin-1 levels in type 2 diabetic patients with ischaemic heart disease (1996)
    Springer
    Acta diabetologica 33 (1996), S. 246-248 
    Details
    ISSN: 1432-5233
    Keywords: Endothelin-1 (Et-1) ; Type 2 diabetes mellitus ; Coronary artery disease (CAD)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To investigate whether circulating endothelin-1 (Et-1) may be related to the increased incidence and severity of ischaemic heart disease in type 2 diabetes mellitus, we compared the concentrations in type 2 diabetic patients and in non-diabetic patients with coronary artery disease (CAD) angiographically documented. Plasma levels of Et-1 were determined in 34 type 2 diabetic patients with CAD (16 with stable angina, 6 with unstable angina, 12 with previous myocardial infarction) and in 19 non-diabetic patients with CAD (4 with stable angina, 5 with unstable angina, 10 with previous myocardial infarction). Fifteen diabetic patients without CAD and 9 healthy volunteers served as control subjects. In the type 2 diabetic patients, the mean Et-1 levels were 3.19±1.61 pmol/l in those with stable angina, 3.58±1.92 pmol/l in those with unstable angina, 4.24±2.53 pmol/l in those with myocardial infarction. These values were not significantly different one another, nor from the values obtained from type 2 diabetic controls (3.64±2.13 pmol/l). In the non-diabetic patients, the mean Et-1 levels were 3.92±0.73 pmol/l in those with stable angina, 4.35±1.67 pmol/l in those with unstable angina, 4.33±1.66 pmol/l in those with myocardial infarction. These values were not significantly different one another, but significantly higher than those obtained from healthy controls (2.07±0.67 pmol/l;P〈0.001). No significant differences were found in Et-1 levels between diabetic and non-diabetic patients with stable, unstable angina and previous myocardial infarction. In contrast, a statistically significant difference was found in Et-1 levels between diabetic and non-diabetic control subjects (P〈0.05). In conclusion, similar raised concentrations of Et-1 in diabetic and non-diabetic patients with stable, unstable angina and previous myocardial infarction do not support the hypothesis that higher levels of Et-1 in diabetic patients are responsible for the increased incidence of CAD in diabetes mellitus. However, the raised Et-1 levels found in diabetic patients in the absence of CAD strongly suggest that a generalised endothelial dysfunction, documented in our study by increased levels of Et-1, most probably precedes subsequent cardiovascular diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02048552
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Mycelial elongation and sporulation of two fungi on amended media in light or dark (1993)
    Springer
    Antonie van Leeuwenhoek 63 (1993), S. 23-27 
    Details
    ISSN: 1572-9699
    Keywords: 6-benzyladenine ; Botrytis allii ; caffeic acid ; chlorogenic acid ; Colletotrichum dematium ; kinetin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Botrytis allii andCollectotrichum dematium are onion pathogens which can infect in the field and cause decay in storage. Some phenolics can hinder development of these fungi, but the effect of cytokinins is not clear. Cytokinins (kinetin or 6-benzyladenine) or phenolics (caffeic or chlorogenic acids) were added to agar at concentrations of 0 to 10−3 M. Cultures were continuously irradiated with fluorescent light or maintained in the dark for 6 days. On unamended media, final mycelial elongation was 45 or 17.8 mm and sporulation was 28 or 10.6 × 104 spores/ml forBotrytis andColletotrichum, respectively. ForBotrytis, mycelial elongation was slightly (5%) but significantly increased and sporulation increased by 21% by incubation on phenolics as compared to cytokinins. Mycelial extension ofColletotrichum was not affected by amendment. Sporulation ofColletotrichum on kinetin was 16 to 28% greater than on the other amendments. As amendments concentration increased elongation of mycelia of both fungi decreased. Sporulation ofBotrytis increased by 60% as amendment concentration increased from 0 to 10−5 M and then decreased 25% at 10−3 M. As amendment concentration increased from 0 to 10−3 M, sporulation ofColletotrichum increased by 45%. Incubation in light increased mycelial extension 3 to 17% forBotrytis andColletotrichum respectively, and sporulation was increased approximately 78% for both fungi. These compounds do not appear to inhibit development of theseBotrytis orColletotrichum species in culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00871727
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of Muskmelon Fruit Peroxidases at Different Developmental Stages (2000)
    Springer
    Biologia plantarum 43 (2000), S. 373-379 
    Details
    ISSN: 1573-8264
    Keywords: Cucumis melo ; chromatography ; electrophoresis ; host-plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An increase in exocarp peroxidase activity was observed in fruit at 5 to 30 days post pollination (DPP), and decreased at 40 and 50 DPP. Total peroxidase activity of the mesocarp was significantly lower than the exocarp in all developmental stages. Mesocarp peroxidase activity decreased consecutively from outer, to middle and, to inner tissue at every developmental stage. Total activity in the mesocarp peaked at 20 DPP. Native-PAGE of exocarp tissue showed at least two cathodic (basic) peroxidases and two anionic (acidic) peroxidases. The number of isozymes was greatest and bands most intense at 30 DPP. IEF-PAGE of the 5 to 50 DPP fruit exocarp showed at least 8 peroxidase isozymes (pI 4.6 to 9.6). Anion exchange chromatography showed only one peak of anionic peroxidase activity that was not evident until 15 DPP. This peak was greatest at 30 DPP and declined at 40 and 50 DPP. Cationic peroxidase isozymes appeared to be the predominant and most intense isoforms throughout fruit development. The changes in peroxidase activity corresponded to fruit formation and may be associated with susceptibility to fruit rot.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026777907099
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    Paper (German National Licenses)
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