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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Computing 44 (1990), S. 1-19 
    ISSN: 1436-5057
    Keywords: 68U05 ; 68C25 ; Polygon ; geodesics ; diameter ; furthest neighbour ; algorithm ; complexity ; computational geometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Gegeben sei ein einfaches PolygonP mitn Ecken. Wir geben einen Algorithmus an, der ein Punktepaar auf der Begrenzung vonP liefert, welches die Länge des kürzesten Weges maximiert, der im Äußeren des Polygons verläuft. Den Weg bezeichnen wir als den äußeren geodätischen Durchmesser vonP. Unser Algorithmus benötigt 0(n 2) Zeit und erfordert 0(n) Speicherplatz. Zu unserer Überraschung ist das Problem von dem, der Berechnung des inneren geodätischen Durchmessers vonP völlig verschieden. Während der innere Durchmesser immer in Ecken vonP endet, muß dies für den äußeren Durchmesser nicht der Fall sein. Schließlich zeigen wir noch, daß der Algorithmus so erweitert werden kann, daß er das Problem der entferntesten äußeren geodätischen Nachbarn löst.
    Notes: Abstract Given a simple polygonP ofn vertices, we present an algorithm that finds the pair of points on the boundary ofP that maximizes theexternal shortest path between them. This path is defined as theexternal geodesic diameter ofP. The algorithm takes0(n 2) time and requires0(n) space. Surprisingly, this problem is quite different from that of computing theinternal geodesic diameter ofP. While the internal diameter is determined by a pair of vertices ofP, this is not the case for the external diameter. Finally, we show how this algorithm can be extended to solve theall external geodesic furthest neighbours problem.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0603
    Keywords: Brain ; Artery ; Cultured smooth muscle cells ; cGMP ; cAMP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cerebral blood vessels have unique properties when compared to most peripheral vascular beds. One such property is that large cerebral vessels are involved in the regulation of cerebral vascular resistance. Studying smooth muscle cells isolated from these vessels will determine how phenotypic properties of these cells contribute to unique cerebrovascular function. Therefore we developed a method of culturing smooth muscle cells from explants of cerebral arteries of porcine brains obtained, gratis, from a local slaughter house. Cells isolated and cultured by the methods described herein were of smooth muscle origin as indicated by histochemical staining for smooth muscle α-actin. Further, we examined the response of the cultured cells to agonists which activate the cGMP dependent vasodilator system by stimulating soluble guanylyl cyclase (nitroglycerin and sodium nitroprusside) or particulate guanylyl cyclase (C-type natriuretic peptide). Also, forskolin activation of adenylyl cyclase was examined. These agents stimulated an increase in intracellular cGMP and cAMP in a manner that was reproducible in every cell isolation (20 brains) and which remained unchanged through nine passages. Additionally, the cells could be frozen, thawed and replated without loss of responsiveness to these agents. The protocol reported here provides a method for culturing cerebral artery smooth muscle cells that is inexpensive, relatively simple, and which yields cells that can be utilized through multiple passages.
    Type of Medium: Electronic Resource
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