ISSN:
1573-5168
Keywords:
cytochrome P-450
;
β-naphthoflavone
;
7-alkoxycoumarin
;
7-methoxycoumarin
;
7-ethoxycoumarin
;
7-propoxycoumarin
;
7-butoxycoumarin
;
fish
;
rainbow trout
;
liver microsomes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Evidence has recently been presented for variation in the inducibility of various 7-alkoxycoumarin-O-dealkylase activities in liver microsomes from a number of mammalian species by β-naphthoflavone (βNF). In the present study we have investigated the inducibility of hepatic microsomal 7-methoxycoumarin-O-demethylase, 7-ethoxycoumarin-O-deethylase, 7-propoxycoumarin-O-depropylase and 7-butoxycoumarin-O-debutylase activities in rainbow trout by βNF. O-demethylase activity was increased approximately 17-fold, O-deethylase and O-depropylase activities approximately 9-fold and O-debutylase activity approximately 25-fold. The kinetics of the various hepatic microsomal 7-alkoxycoumarin-O-dealkylase activities were investigated in control and βNF-treated rainbow trout. The O-demethylase-, O-depropylase- and O-debutylase activities exhibited monophasic Michaelis-Menten kinetics in liver microsomes from both control and βNF-treated rainbow trout, whereas the O-deethylase activity exhibited biphasic Michaelis-Menten kinetics in control liver microsomes and monophasic Michaelis-Menten kinetics in liver microsomes from βNF-treated rainbow trout.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01875022
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