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Identification of type 1 and type 2 light-harvesting chlorophyll a/b-binding proteins using monospecific antibodies

Sigrist, M. ; Staehelin, L.A.

Amsterdam : Elsevier

Biochimica et Biophysica Acta (BBA)/Bioenergetics 1098 (1992), S. 191-200

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ISSN: 0005-2728

Keywords: (Petunia ; Anti peptide antibody ; CAB gene ; Light harvesting complex ; Photosystem II ; Spinach ; Thylakoid membrane ; Tomato) ; [abr] -LHC II; light-harvesting complex associated with Photosystem II ; [abr] 2-amino-2-methyl-1,3-propanediol ; [abr] AA; amino acid ; [abr] BSA; bovine serum albumin ; [abr] ELISA; enzyme-linked immuno sorbent assay ; [abr] Hepes; 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid ; [abr] KLH; keyhole limpet hemocyanin ; [abr] LHCP; light-harvesting chlorophyll a/b-binding protein ; [abr] OEC; oxygen-evolving complex ; [abr] PAGE; polyacrylamide gel electrophoresis ; [abr] PBS; phosphate-buffered saline ; [abr] PS; Photosystem ; [abr] SDS; sodium dodecyl sulfate ; [abr] SMCC; N-succimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Biology , Chemistry and Pharmacology , Medicine , Physics

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1016/S0005-2728(05)80336-6

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Analysis of liquid surface films by pulsed laser photoacoustic spectroscopy (1987)

Sigrist, M. W. ; Chen, Z. H.

Springer

Applied physics 43 (1987), S. 1-7

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ISSN: 1432-0649

Keywords: 68.15.+e ; 78.65.−s ; 78.20.Nv

Source: Springer Online Journal Archives 1860-2000

Topics: Physics

Notes: Abstract The photoacoustic generation of plane acoustic waves in strongly absorbing or opaque liquids by pulsed laser radiation is discussed both experimentally and theoretically. The regimes of a confined and a free surface of the liquid are considered. The model which takes the temporal shape of the laser pulses applied in the experiments into account, implies that spectroscopic studies are feasible with direct photoacoustic generation and detection also for opaque liquids. The experiments are performed with a tunable hybrid CO2 laser and piezoelectric detection. For the first time liquid/liquid interfaces are studied by this technique. We demonstrate that the presence of an absorbing liquid film with a thickness of 〉1 μm on the surface of another liquid amplifies the acoustic signal which is detected in the bottom liquid. The enhancement depends on the thickness and the optical and thermal properties of the film medium. The surface layer can be analyzed on the basis of the photoacoustic spectrum. It is also shown that this non-contact method is surface-film selective and should thus prove useful for pollution analysis of liquid surfaces.