ISSN:
1617-4623
Keywords:
Site-specific mutagenesis
;
Oxidative DNA damage
;
8-Hydroxyguanine
;
Mutator
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Oxidative damage to guanine in DNA results in the formation of 8-oxoguanine, which has been shown to induce G → T transversions targeted to this site. The mutagenicity of this lesion was studied in several mutator strains of Escherichia coli, using single-stranded DNA containing a single 8-oxoguanine residue. The frequencies of targeted G → T transversions increased markedly in mutY strains, while this mutagenic event was not affected in mutM or mutS strains. Introdution of a mutM mutation into a mutY strain caused a somewhat higher frequency of G → T transversions than that in the mutY strain and the effect of a mutS mutation was marginal. We conclude that the mutY gene plays a crucial role in preventing targeted G → T mutations derived from misreplication of the 8-oxoguanine-containing template DNA.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00281603
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