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  • 1
    Electronic Resource
    Electronic Resource
    Single copy DNA homology in sea stars (1982)
    Springer
    Journal of molecular evolution 18 (1982), S. 92-101 
    Details
    ISSN: 1432-1432
    Keywords: Sequence homology ; DNA ; Genome evolution ; Starfish DNA ; Molecular phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sequence homology in the single copy DNA of sea stars has been measured. Labeled single copy DNA fromPisaster ochraceus was reannealed with excess genomic DNA fromP. brevispinus, Evasterias troschelii, Pycnopodia helianthoides, Solaster stimpsoni, andDermasterias imbricata. Reassociation reactions were performed under two criteria of salt and temperature. The extent of reassociation and thermal denaturation characteristics of hybrid single copy DNA molecules follow classical taxonomic lines.P. brevispinus DNA contains essentially all of the sequences present inP. ochraceus single copy tracer whileEvasterias andPycnopodia DNAs contain 52% and 46% of such sequences respectively. Reciprocal reassociation reactions with labeledEvasterias single copy DNA confirm the amount and fidelity of the sequence homology. There is a small definite reaction of uncertain homology betweenP. ochraceus single copy DNA andSolaster orDermasterias DNA. SimilarlySolaster DNA contains sequences homologous to approximately 18% ofDermasterias unique DNA. The thermal denaturation temperatures of heteroduplexes indicate that the generaPisaster andEvasterias diverged shortly after the divergence of the subfamilies Pycnopodiinae and Asteriinae. The twoPisaster species diverged more recently, probably in the most recent quarter of the interval since the separation of the generaPisaster andEvasterias.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01810827
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  • 2
    Electronic Resource
    Electronic Resource
    Detection of furocoumarins in plants and plant products with an ultrasensitive biological photoassay employing a DNA-repair-deficient bacterium (1986)
    Springer
    Journal of chemical ecology 12 (1986), S. 915-932 
    Details
    ISSN: 1573-1561
    Keywords: Furocoumarins ; psoralen ; 5-methoxypsoralen ; 8-methoxypsoralen ; phototoxicity ; photobiological assay ; celery ; carrot ; analysis ; umbelliferous seeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The application of an ultrasensitive photobiological assay which detects photosensitizing furocoumarins with sensitivities as high as 1 × 10−11 g is discussed in relation to these molecules as phytoalexins. Examples of the utilization of this technique, verified by both HPLC and TLC, are the analyses of healthy and diseased celery and carrots, dry seeds, plant extracts and oils, and whole plants and leaves. The usefulness of this method in following the metabolic detoxification of furocoumarins is also illustrated. The extreme sensitivity of the test has permitted the detection, for the first time, of both 5-methoxypsoralen and 8-methoxypsoralen in fresh carrot roots.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01020261
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
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