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  • 82.65.Pa  (1)
  • Key words Bioluminescence  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied physics 61 (1995), S. 511-517 
    ISSN: 1432-0630
    Keywords: 68.35.Ja ; 82.20.Kh ; 82.65.Pa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract In this short review we consider some selected results for the dissociation of hydrogen on metal surfaces. In particular we focus our attention on the effects that surface corrugation have on the dissociation and also how rotations and vibrations couple in the interaction region. Results from time dependent quantum wavepacket calculations have been particularly helpful in forming our ideas of how diatomics dissociate. We describe this method along with two examples of propagation schemes. The power of limited dimensional models to rationalize experimental observations is clearly demonstrated.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1617-4623
    Keywords: Key words Bioluminescence ; Chlamydomonas ; Chloroplast transformation ; Chloroplast translation ; Vital reporter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The use of luciferases as reporters of gene expression in living cells has been extended to the chloroplast genome. We show that the luciferase from the soft coral Renilla reniformis (Rluc) can be successfully expressed in the chloroplast of Chlamydomonas reinhardtii. Expression of the rluc cDNA was driven by the promoter and 5′ untranslated regions of the atpA gene. Western analysis with an anti-Rluc antibody detected a single polypeptide of 38 kDa in the luminescent cells. This is 3 kDa larger than native Rluc, and suggests that translation of the chimeric mRNA begins at the atpA start codon, 29 codons upstream from the rluc start site. We also show that the luminescence of the transformants was sufficient to enable imaging of colonies using a cooled CCD camera.
    Type of Medium: Electronic Resource
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