ISSN:
1619-7089
Keywords:
Lymphocytes
;
99mTc
;
111In
;
Stannous
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The biodistribution patterns of 99mTc (99mTc-lymph) and 111In-lymphocytes with [111In-(Sn)-lymph] or without (111In-lymph) stannous ion treatment was compared in Lewis rats. Syngeneic lymphocytes were labeled with either 125 μCi (4.63 MBq) 99mTc or 5 uCi (1985 kBq) 111In per 2×107 cells. Mean labeling efficiency for 99mTc and 111In was 68.61%±3.90% (SEM) and 87.22%±2.01% (SEM) respectively. 99mTc-lymph (n=4), 111In-lymph (n=6) and 111In-(Sn)-lymph (n=6) rats received 2x107 cells and were killed 18 h later. While 99mTc-lymph demonstrated significantly less localization in spleen, lymph nodes, and blood (P(F)0.01) as compared with 111In-lymph, 111In-(Sn)lymph also demonstrated a significant difference (P[F]=0.0001) in lymph node accumulation when compared to 111In-lymph. As the activity levels utilized are not associated with cell radiation damage, these alterations in biodistribution do not reflect viability or chromosomal damage, but appear related to stannous ion exposure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00253795
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