ISSN:
1279-8509
Keywords:
ABMT
;
PBSCT
;
Allogeneic BMT
;
Lymphocyte subsets
;
NK cells
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Abstract. A variety of T, B and natural killer (NK) cell subsets defined by surface markers were analyzed by double immunofluorescence flow cytometry in the peripheral blood of patients following autologous bone marrow transplantation (ABMT, n=14), autologous peripheral blood stem cell transplantation (PBSCT, n=10) and allogeneic bone marrow transplantation (allo-BMT, n=6). Patients following ABMT were divided in 2 groups, those who did not received G-CSF post-transplant (ABMT, n=6) and those who did (ABMT+G, n=8). All patients following PBSCT or allo BMT received G-CSF. In all the groups prolonged significant decreases with respect to normal numbers were observed for the T CD3+, CD2+ and CD25+ subsets, more profound for the CD4+ subset but less for the CD8+ subset, especially following PBSCT (only decreased at 1 month). A significant expansion of the CD3+CD57+ and CD8+CD57+ phenotypes was noticed between 9 and 12 months following ABMT, the group of longer follow-up. Long-lasting expansion of the NK-like CD3+CD56+ and CD3+CD16+ subsets was also observed. The B CD19+ and CD20+ subsets had a significant overexpression from 4 months after ABMT, showing a normally balanced Igk+ : Ig1+ ratio. Concordantly, the HLA-DR+ and HLA-DQ+ subsets showed significant increases. The NK CD56+ and CD16+ subsets had a faster recovery than the T or B subsets in all the groups. However, the CD3-CD56+, CD3-CD16+, CD16+CD56+, CD3-CD8+, and especially the CD3-CD57+, CD16+CD57+, and CD56+CD57+ subsets had a slower recovery than the global CD56+, CD16+, or CD57+ subsets. The biological and clinical implications of these findings are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s00282-997-0301-3
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