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  • 1
    ISSN: 1432-0983
    Keywords: Yeast ; Open reading frames ; Database ; Genetic nomenclature ; Codon bias ; Duplicated genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The amount of nucleotide sequence data is increasing exponentially. We therefore continued our effort to make a comprehensive database for the yeast Saccharomyces cerevisiae. In this database (ListA2) we have compiled 1001 protein coding sequences from this organism. Each sequence has been attributed a single genetic name and in the case of allelic duplicated sequences, synonyms are given, if necessary. For the nomenclature we have introduced a standard principle for naming gene sequences based on priority rules. We have also applied a simple method to distinguish duplicated sequences of one and the same gene from non-allelic sequences of duplicated genes. By using these principles we have sorted out a lot of confusion in the literature and databanks. Along with the genetic name, the mnemonic from the EMBL databank, the codon bias, reference of the publication of the sequence and the EMBL accession numbers are included for each entry. The database is available on request.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Current genetics 24 (1993), S. 472-480 
    ISSN: 1432-0983
    Keywords: ADE2 ; β-Galactosidase ; Gene expression ; GCN4 ; Promoter ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regulation of ADE2 gene expression was investigated in the yeast S. cerevisiae using translational fusions between this gene and the lacZ gene from E. coli. Expression was repressed in the presence of adenine and slightly increased under amino-acid starvation conditions. The promoters of the ADE2 gene, and of other genes involved in adenine biosynthesis, contain the hexanucleotide sequence TGACTC. A search for the hexanucleotide TGACTC in yeast promoter sequences revealed that many genes not related to amino-acid biosynthesis contain such sequences. We show here that these elements play a crucial role in ADE2 regulation since mutations in two such elements drastically reduced gene expression. Maximal expression required the transcriptional activators Bas1, Bas2 and Gcn4, whereas Yap1 had only minor effects.
    Type of Medium: Electronic Resource
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