Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Polymer and Materials Science  (2)
  • AFLP  (1)
  • Inheritance  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry (1991)
    Springer
    European journal of pediatrics 150 (1991), S. 161-165 
    Details
    ISSN: 1432-1076
    Keywords: Chronic granulomatous disease ; Dihydrorhodamine 123 ; Diagnosis ; Inheritance ; Flow microcytofluorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Dihydrorhodamine 123 (DHR) attached to membranes of granulocytes (PMN) and monocytes is caused to fluoresce by reactive oxygen intermediates (ROI) indicating the ability of phagocytes to produce these microbicide metabolites in a flow microcytofluorimeter. Whole blood samples from five boys with known chronic granulomatous disease (CGD) and from their mothers (and from one father and one grandmother), were examined following erythrocyte lysis in order to test this new method. An incubation period of 10 min with phorbol-myristate-acetate, followed by another 15 min incubation period with DHR before flow microcytofluorimetric analysis of 5 or 10×103 phagocytes, was sufficient to obtain the following results. PMN and monocytes from four patients with CGD could clearly not produce any ROI whereas cells from one patient displayed decreased activity in ROI production as compared to cells from a healthy donor. The X-linked mode of inheritance was detected in six carriers by the presence of two different cell populations (one normal ROI-producing and one negative or less active population). All the phagocytes from one mother produced ROI in normal amounts suggesting an autosomal mode of inheritance. All in all, the method presented provides a fast and most simple tool to diagnose CGD, to determine a decrease or total lack of ROI production and to establish the mode of inheritance of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01963557
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Bruchverhalten eines vergüteten und eines kaltgezogenen Stahles im Gleichgewichtszustand mit Druckwasserstoff (1983)
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 14 (1983), S. 284-288 
    Details
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Fracture of Tempered and Cold Drawn Steel in Equilibrium with Compressed HydrogenTensile specimens are surface activated and stored in compressed hydrogen until equilibrium is attained. Charged and uncharged reference specimens are tested in air and in compressed hydrogen. The embrittlement of colddrawn steel (plenty of traps) is independent of strain rate and is caused mainly by the prior charging. Tempered steel (fewer traps) is not embrittled by charged hydrogen but by the effect of external hydrogen during the test. The degree of embrittlement decreases with increasing strain rate. In all cases of embrittlement there is in addition a strong notch sensitivity.
    Notes: Zugproben werden oberflächenaktiviert und solange in komprimiertem Wasserstoffgas gelagert, bis sich Gleichgewicht eingestellt hat. Beladene und unbeladene Proben werden an Luft und in komprimiertem Wasserstoff zerrissen. Die Versprödung der fallenreichen kaltgezogenen Proben ist kaum von der Verformungsgeschwindigkeit abhängig und beruht hauptsächlich auf der vorherigen Beladung. Die fallenarmen vergüteten Proben verspröden nicht durch vorherige Beladung sondern durch den Wasserstoff, der während des Zugversuches einwirkt. Diese Versprödung wird mit wachsender Verformungsgeschwindigkeit geringer. Zusammen mit der Wasserstoffversprödung tritt in beiden Fällen starke Kerbempfindlichkeit auf.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mawe.19830140808
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Zur Plastoermüdung von Stahl unter dem Einfluß von Druckwasserstoff (1979)
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 10 (1979), S. 57-59 
    Details
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Low Cycle Fatigue of Steel in Hydrogen EnvironmentThe low cycle fatigue fracture of a medium strength tempered steel ist studied in compressed hydrogen resp. nitrogen (150 bar). For given strain amplitudes, hydrogen reduced strongly the cycles to failure. Probably the crack growth is accelerated rather than the crack initiation. The frequency had no influence on the number of cycles to failure.
    Notes: Es wird über den Einfluß von Druckwasserstoff auf das Bruchverhalten eines Vergütungsstahles (Werkstoff-Nr. 1. 7720) bei Belastung im Low Cycle Fatigue Bereich berichtet. Versuche unter H2 und N2 von jeweils 150 bar zeigten beim Wasserstoff eine erhebliche Abnahme der Lastwechselzahl bis zum Bruch. Ursache ist wahrscheinlich die erhöhte Rißausbreitungs-geschwindigkeit. Eine Frequenzabhängigkeit der Lebensdauer wurde nicht festgestellt.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mawe.19790100209
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Reproducibility testing of RAPD, AFLP and SSR markers in plants by a network of European laboratories (1997)
    Springer
    Molecular breeding 3 (1997), S. 381-390 
    Details
    ISSN: 1572-9788
    Keywords: DNA markers ; RAPD ; AFLP ; SSR ; microsatellite ; network ; reproducibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A number of PCR-based techniques can be used to detect polymorphisms in plants. For their wide-scale usage in germplasm characterisation and breeding it is important that these marker technologies can be exchanged between laboratories, which in turn requires that they can be standardised to yield reproducible results, so that direct collation and comparison of the data are possible. This article describes a network experiment involving several European laboratories, in which the reproducibility of three popular molecular marker techniques was examined: random-amplified fragment length polymorphism (RAPD), amplified fragment length polymorphism (AFLP) and sequence-tagged microsatellites (SSR). For each technique, an optimal system was chosen, which had been standardised and routinely used by one laboratory. This system (genetic screening package) was distributed to different participating laboratories in the network and the results obtained compared with those of the original sender. Different experiences were gained in this exchange experiment with the different techniques. RAPDs proved difficult to reproduce. For AFLPs, a single-band difference was observed in one track, whilst SSR alleles were amplified by all laboratories, but small differences in their sizing were obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009612517139
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...