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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 428 (1994), S. 415-417 
    ISSN: 1432-2013
    Keywords: Heart ; Cardiac muscle ; Contraction ; E-C coupling ; Calcium channels ; Ryanodine receptors ; Intracellular calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This paper examines the [Ca2+]i transient in isolated rat heart cells using a laser scanning confocal microscope and the calcium indicator fluo-3. We find that the depolarization-evoked [Ca2+]i transient is activated synchronously near the surface and in the middle of the heart cell with similar kinetics of activation. The time of rise of the transient did not depend on whether the sarcoplasmic reticulum (SR) Ca-release was abolished (by thapsigargin and ryanodine). The synchrony of activation and the similarity of levels of [Ca2+]i at the peripheral and deeper myoplasm (regardless of the availability of SR Ca-release) shows that sarcolemmal Ca channels and SR Ca-release channels are distributed throughout the rat heart cell and that the propagation of the action potential into the interior of the cell is rapid. In addition, the activation of calcium release from the SR by CICR is rapid (≪2 ms) when compared to the time-course of calcium influx via the sarcolemmal Ca channel.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 97 (1987), S. 3-13 
    ISSN: 1573-5036
    Keywords: AN value ; Isotope dilution ; 15N ; N balance ; N2 fixation ; Soil nitrogen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Accurate estimates of N2 fixation by legumes are requisite to determine their net contribution of fixed N2 to the soil N pool. However, estimates of N2 fixation derived with the traditional15N methods of isotope dilution and AN value are costly. Field experiments utilizing15N-enriched (NH4)2SO4 were conducted to evaluate a modified difference method for determining N2 fixation by fababean, lentil, Alaska pea, Austrian winter pea, blue lupin and chickpea, and to quantify their net contribution of fixed N2 to the soil N pool. Spring wheat and non-nodulated chickpea, each fertilized with two N rates, were utilized as non-fixing controls. Estimates of N2 fixation based on the two control crops were similar. Increasing the N rate to the controls reduced AN values 32, 18 and 43% respectively in 1981, 1982 and 1983 resulting in greater N2 fixation estimates. Mean seasonal N2 fixation by fababean, lentil and Austrian winter pea was near 80 kg N ha−1, pea and blue lupin near 60 kg N ha−1, and chickpea less than 10 kg N ha−1. The net effects of the legume crops on the soil N pool ranged from a 70 kg N ha−1 input by lentil in 1982, to a removal of 48 kg N ha−1 by chickpea in 1983. Estimates of N2 fixation obtained by the proposed modified difference method approximate those derived by the isotope dilution technique, are determined with less cost, and are more reliable than the total plant N procedure.
    Type of Medium: Electronic Resource
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