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  • ATP  (1)
  • DNA-drug Complex  (1)
  • DNA-protein interaction  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The cofactor ATP in DNA-RecA complexes is not intercalated between DNA bases (1994)
    Chicester [u.a.] : Wiley-Blackwell
    Journal of Molecular Recognition 7 (1994), S. 221-226 
    Details
    ISSN: 0952-3499
    Keywords: RecA protein ; DNA-protein interaction ; Linear dichroism ; Intercalation ; ATP ; Homologous recombination ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: In an attempt to understand the role of ATP as a cofactor at the interaction of the RecA protein with DNA, we have studied the orientation geometries of the cofactor analogs adenosine 5′-O-(3-thiotriphosphate) (ATPγS) and guanosine 5′-O-(3-thiotriphosphate) (GTPγS) in RecA-DNA complexes using flow linear dichroism spectroscopy. Both cofactors promote the formation of RecA-DNA complexes of similar structure as judged from similar orientations of DNA bases. The DNA orientation was probed through the dichroism of the long-wavelength absorption of a DNA analog, poly(dεA). In this way differences between the dichroic spectra of the ATPγS-RecA-DNA and GTPγS-RecA-DNA complexes, observed in the shorter-wavelength region, are related to orientation at variations of the cofactor chromophores. The results show that the guanine plane of GTPγS is oriented parallel with the principal axis of the complex in contrast to the more perpendicular orientation of the DNA bases. This observation directly excludes the possibility that the cofactor could be intercalated between the DNA bases. This observation directly excludes the possibility that the cofactor could be intercalated between the DNA bases. The orientation of the adenine base of ATPγS, which may be similar to that of guanine of GTPγS albeit not exactly the same, is also inconsistent with intercalation. The possibility that the cofactor bound to the protein could be intercalated in DNA had been speculated from the observation that some DNA intercalators can induce RecA binding to DNA in the absence of cofator. There are probably no direct interactions between the cofator and the DNA bases and the role of the cofactor is probably related to interaction with RecA and a modification of protein conformation.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmr.300070311
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Analysing DNA complexes by circular and linear dichroism (1994)
    Chicester [u.a.] : Wiley-Blackwell
    Journal of Molecular Recognition 7 (1994), S. 141-155 
    Details
    ISSN: 0952-3499
    Keywords: DNA binding geometries ; DNA-drug Complex ; DNA-protein complex ; Circular dichroism ; Linear dichroism ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The application of linear and circular dichroism (LD and CD) in nucleic acid research id illustrated by recent results aimed at answering specific structural problem in the interaction of DNA with molecules of biological importance. We first consider the circumstances under which ligands, such as DAPI (4′, 6-diamidino-2-phenylindole), change their preferred binding mode in the minor groove to major groove binding or intercalation. As an extension of this problem we refer to the switch between groove binding and intercalation of structurally similar ligands such as ellipticines and trigonal ruthenium complexes. We also explore the use of LD and CD in the determination of the structure of the complex formed between the polynucleotide poly(dA) and the novel ‘peptide nucleic acid’, consisting of nucleic acid bases joined by a polyamide homomorphous with the deoxyribose-phosphate backbone of DNA. Finally, the structure and interaction of the recombination enzyme RecA with DNA is discussed, in particular the influence of the presence of the intercalators, groove binders or covalent DNA adducts.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmr.300070211
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    Paper (German National Licenses)
    Fulltext
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