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  • 1
    Electronic Resource
    Electronic Resource
    Relaxation and force during fatigue and recovery of the human quadriceps muscle: relations to metabolite changes (1991)
    Springer
    Pflügers Archiv 418 (1991), S. 153-160 
    Details
    ISSN: 1432-2013
    Keywords: ATP ; Electrical stimulation ; Force ; Phosphate ; Phosphocreatine ; Relaxation ; Skeletal muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Force and relaxation were measured during electrical stimulation of the quadriceps muscle of 14 volunteers. Stimulation produced 51.2 s of intermittent ischaemic contractions either as 16 3.2-s tetani or as 64 0.8-s tetani. Changes during recovery were followed for 180 s. On 8 subjects muscle biopsies were taken during work and after the rest period for determination of ATP, phosphocreatine and intermediates in glucolysis. The stimulation using 0.8-s contractions gave more pronounced fatigue and slowing of relaxation. There was a good correlation between force and relaxation during work but this relation changed during recovery, indicating that no general relation exists between these two contraction characteristics. In the 0.8-s stimulation more ATP was utilized and there were more profound changes in metabolite levels. We found a correlation between estimated [H2PO 4 − ] and relaxation covering both work and recovery and hypothesize that inorganic phosphate and its removal by phosphocreatine resynthesis during recovery might be important. Since stimulation patterns differ in force and relaxation even after the recovery period we suggest that additional factors, such as pH, are of importance in this work model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00370464
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  • 2
    Electronic Resource
    Electronic Resource
    A luminometric method for the determination of ATP and phosphocreatine in single human skeletal muscle fibres (1991)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 6 (1991), S. 123-129 
    Details
    ISSN: 0884-3996
    Keywords: ATP ; luminescence ; phosphocreatine ; single fibres ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: A sensitive method for the analysis of ATP and phosphocreatine (PCr) in single human skeletal muscle fibres is described. Muscle tissue was freeze-dried and single fibres were dissected free with the aid of low-power microscopy. The fibres were then extracted in trichloroacetic acid and neutralized with KHCO3. The assay is based on the continuous monitoring of light produced as a result of ATP degradation in the firefly luciferase reaction. PCr is measured as the amount of ATP formed in the creatine kinase reaction. The coefficient of variation was less than 4% for both ATP and PCr determination. The amount of tissue required for the assay is approximately 0.5 μg (dry weight). The assay showed good agreement with spectrophotometric and high-performance liquid chromatographic (HPLC) measurements made upon extracts of whole muscle tissue.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170060210
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    Paper (German National Licenses)
    Fulltext
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