ISSN:
1432-072X
Keywords:
Chlorella fusca
;
Continuous turbidostatic culture
;
CO2-assimilation
;
ATP-level
;
Energy charge
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In continuous cultures of Chlorella fusca under steady state conditions, the CO2-fixation rate, the ATP-level, the apparent rate of photophosphorylation as calculated from the changes in the ATP-level during light to dark or dark to light transients and the energy charge were measured at various environmental conditions. During growth the energy charge was around 0.64. CO2-assimilation and the apparent ATP-synthesis were strongly dependant on light intensity, however the ATP-level was independant on it. Since the rates of apparent ATP-synthesis and of the CO2-fixation do not seem to be strictly correlated in a logic way when environmental factors are changed and furthermore the stoichiometry of 3 ATP necessary per CO2 fixed was never achieved, the described method frequently used for procaryotes to determine the in vivo rate of phosphorylation does not give valid results in highly compartimented eukaryotic cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00406032
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