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  • AV delay optimization  (1)
  • Affinity labeling Biopolymer dynamics Reactive dinucleotide derivative  (1)
  • Antisense oligodeoxynucleotides  (1)
  • Antisense oligonucleotide: Nucleic acid structure  (1)
  • 1
    ISSN: 0014-5793
    Keywords: Antisense oligonucleotide: Nucleic acid structure
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0014-5793
    Keywords: Affinity labeling Biopolymer dynamics Reactive dinucleotide derivative
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    ISSN: 1435-2451
    Keywords: Key words Pancreatic cancer ; p53 ; Antisense oligodeoxynucleotides ; Cell culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Introduction: Pancreatic adenocarcinomas are largely resistant to apoptosis. More than 50% of pancreatic tumors reveal mutations in the p53 tumor suppressor gene. Methods: We investigated the growth of pancreatic tumor cells after downregulation of p53 protein expression by antisense oligodeoxynucleotides. Results: Proliferation and p53 expression of PancTu-I cells overexpressing mutant p53 protein were inhibited by antisense oligodeoxynucleotide treatment. When analyzed, two of three other pancreatic tumor cell lines with mutated p53 were also inhibited in their growth. Two of two wild-type (wt) p53 pancreatic tumor cells were not significantly influenced by p53 expression and were, only to a lesser extent, affected in their proliferation. K562 cells (lacking p53 mRNA) and normal human skin fibroblasts used as a target mismatch control showed no changes in proliferation rates with treatment. The different biological effects in the various cells were not caused by differences in the uptake of the oligodeoxynucleotides as monitored by confocal laser-scanning microscopy. Conclusions: Truncation and 5′- and 3′-lipophilic modifications of the oligodeoxynucleotides drastically enhanced the growth inhibition of PancTu-I cells, which were resistant to apoptosis-inducing agents. Furthermore, a higher sequence-specificity of the observed effects was achieved with these compounds.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1435-1544
    Keywords: Key words DDD pacing ; AV delay optimization ; Interatrial conduction time ; telemetry ; sense-event marker ; Schlüsselwörter DDD-Stimulation ; AV-Delay-Optimierung ; Interatriale Leitungszeit ; Telemetrie ; Sensing-Marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Hintergrund: Bei DDD-Schrittmacherträgern wird das optimale AV-Delay wesentlich durch die interindividuell unterschiedliche interatriale Leitungszeit bestimmt. Wird diese gemessen, kann durch Addition eines bekannten, mittleren, optimalen Intervalles von 70ms zwischen linksatrialer Depolarisation und ventrikulärem Stimulus ein individuell optimales AV-Delay für VDD- und DDD-Betrieb approximiert werden. Da moderne Schrittmacher über eine Telemetrie verfügen, ist es naheliegend, durch Kombination von rechtsatrialer Telemetrie und linksatrialer Elektrographie (via Ösophagusableitung) vorhandene Programmeroptionen zur individuellen AV-Delay-Programmierung zu nutzen. Studienziele: 1. Prüfung der Eignung rechtsatrialer telemetrischer Elektrogramme oder Sensingmarker als rechtsatriale Referenz. 2. Prüfung der Realisierbarkeit einer Implementierung der linksatrialen Elektrographie in handelsübliche Programmer. Methoden: 1. Mittels eines computerisierten Herzsimulators wurde die Verzögerung telemetrischer atrialer Elektrogramme und Sensingmarker bei 12 Schrittmachern 9 unterschiedlicher Hersteller untersucht. 2. Zur Messung interatrialer Leitungszeiten mit Programmiergeräten wurde die Simultandarstellung des rechtsatrialen Elektrogramms bzw. Markerkanals und der linksatrialen Ableitung mittels externer Hardware- bzw. programmerinterner Softwarefilterung der Ösophagusableitung realisiert. Ergebnisse: Abhängig von der Größe des telemetrischen Delays lassen sich Schrittmachersysteme mit Echtzeittelemetrie (Delay 0-12ms) und Orientierungstelemetrie (Delay 12ms) unterscheiden. Es ließ sich zeigen, daß mit der vorgeschlagenen Implementierung linksatrialer Elektrogramme interatriale zeitliche Relationen sowohl unter Vorhofstimulation als auch bei Vorhofführung an Systemen mit Echtzeittelemetrie bequem erfaßt werden können. Die Voraussetzungen für eine individuelle Programmierung des AV-Delay am Programmiergerät und die automatische Bereitstellung von Programmiervorschlägen sowohl für das Basis- als auch des frequenzmodulierten AV-Delay sind damit erfüllt. Schlußfolgerung: Durch Implementierung einer linksatrialen Ableitung in Schrittmacherprogrammer können interatriale Leitungszeiten leicht erfaßt und die physiologische AV-Delay-Programmierung in der Routine vereinfacht werden.
    Notes: Background: In patients with DDD pacing systems, optimal AV delay is mainly determined by interindividually differing interatrial conduction time. If the latter is measured, individual optimal AV delay in VDD and DDD operation can be approximated by adding a representative, optimal interval of 70ms between left atrial depolarization and ventricular stimulus. In modern pacemakers providing telemetry, combination of right atrial telemetry and left atrial electrography via an esophageal lead could be used to measure interatrial conduction times for individual AV delay programming. Aims of the study: 1. To test the usefulness of telemetric right atrial electrograms and markers as a right atrial measuring reference. 2. To prove the possibility of implementing left atrial electrography into commercial programers. Methods: 1. Using computerized heart simulator, delays of the telemetric electrogram and markers were investigated in 12 DDD systems of 9 different companies. 2. To measure interatrial conduction times with programers, simultaneous recordings of right atrial electrogram and marker channel, resp., and the left atrial electrogram were performed using external hardware or internal software filtering of the esophageal lead. Results: Depending on the range of marker delay, pacing systems with real-time telemetry (delay 0–12ms) and orienting telemetry (delay 12ms) were found. Facilitating left atrial electrogram recording into programers, interatrial time intervals can be measured in DDD and VDD operation in pacing systems with real-time telemetry. These results are prerequisits for individual AV delay programming with the programer. Then both can automatically be proposed, basic AV delay and the frequency modulated AV delay. Conclusion: Facilitating left atrial electrogram recording into pacemaker programers, interatrial conduction times can easily be measured for simplified physiologic AV delay programming during routine check-up.
    Type of Medium: Electronic Resource
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