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  • AVP  (1)
  • Fissure-in-ano  (1)
  • Manometry  (1)
  • bromouridine  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Unsuspected sphincter defects shown by anal endosonography after anorectal surgery (1995)
    Springer
    Diseases of the colon & rectum 38 (1995), S. 249-253 
    Details
    ISSN: 1530-0358
    Keywords: Anus ; Ultrasonography ; Manometry ; Fecal incontinence ; Surgery ; Hemorrhoids ; Fistula ; Fissure-in-ano
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract PURPOSE: Anorectal surgery can lead to fecal soiling and incontinence. Whether surgery changes the anatomy and causes symptoms is unknown. Anatomic changes can be visualized by anal endosonography. METHODS: We studied 50 patients after hemorrhoidectomy (24), fistulectomy (18), and internal sphincterotomy (8). Symptoms were assessed, and anal endosonography, anal manometry, mucosal electrosensitivity, and neurophysiologic tests were performed. RESULTS: In 23 (46 percent) patients, a defect of the anal sphincter was found (13 patients had an internal sphincter defect, 1 had an external sphincter defect, and 9 had a combined sphincter defect), 3 after hemorrhoidectomy, 13 after fistulectomy, and 7 after internal sphincterotomy. Seven patients had symptoms, and they all had a sphincter defect. In the other 16 of 23 patients (70 percent), the sphincter defect did not produce symptoms. An internal sphincter defect lowered maximum basal pressure and shortened sphincter length. CONCLUSION: Anal endosonography can reveal sphincter defects after anorectal surgery. Seventy percent of the patients in this group had no complaints; therefore, defects were unsuspected. This has clinical implications in the evaluation of patients with fecal incontinence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02055596
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Vasopressin accelerates protein synthesis in neonatal rat cardiomyocytes (1999)
    Springer
    Molecular and cellular biochemistry 195 (1999), S. 183-190 
    Details
    ISSN: 1573-4919
    Keywords: AVP ; cardiomyocyte ; cytosolic free calcium ([Ca2+]i) ; protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract Arginine vasopressin (AVP) has been shown to promote vascular smooth muscle cell hypertrophy and hyperplasia of fibroblasts. The present study examines the effect of AVP and endothelin-1 (ET-1) on protein, DNA, and RNA synthesis in primary cultures of serum deprived neonatal rat cardiomyocytes (RC) as assessed by changes in [3H] phenylalanine, [3H] thymidine, and [14C] uridine incorporation respectively. Both AVP and ET-1 evoked significant increases in protein synthesis in RC of 36 ± 12% (p 〈 0.05) and 53 ± 22% (p 〈 0.01) respectively. The stimulating action of AVP on [3H] phenylalanine incorporation was abolished by pretreatment with 2-nitro-4carboxyphenyl-N, N-diphenylcarbamate (NCDC), a phospholipase C (PLC) inhibitor. [14C] uridine incorporation was significantly higher in cells incubated with ET-1 (95 ± 12%) but not AVP (9 ± 11%). Neither AVP nor ET-1 significantly affected cell number or [3H] thymidine incorporation, suggesting a lack of a hyperplastic effect. AVP evoked an increase in [Ca2+]i levels (162 ± 12 nmol/L from a basal value of 77 ± 6 nmol/L) which was completely abolished by pretreatment with either NCDC or cyclopiazonic acid (sarcoplasmic reticulum (SR) Ca2+ pump inhibitor) but unaffected by ryanodine (ryanodine sensitive SR Ca2+ store depletor). Taken together, these data suggest that AVP, in a PLC dependent manner, stimulates both protein synthesis and augments [Ca2+]i release in RC from ryanodine insensitive (IP3 sensitive) Ca2+ stores. Thus, AVP may promote cardiac hypertrophy via direct effects on cardiomyocyte protein synthesis secondary to IP3 mediated [Ca2+]i release.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006961330375
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    In vitro splicing of pre-mRNA containing bromouridine (1994)
    Springer
    Molecular biology reports 19 (1994), S. 109-113 
    Details
    ISSN: 1573-4978
    Keywords: bromouridine ; nucleotide analogue ; pre-mrna ; polypyrimidine tract ; splicing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The artificial UTP-analogue 5-bromouridine 5′-triphosphate (BrUTP) has been used to label pre-mRNAin vitro andin vivo [1, 2]. We have investigated the effect of bromouridine (BrU) in pre-mRNA on the efficiency of splicing. An adenovirus major late II construct was used to prepare four different transcripts, each containing a different amount of BrU. These four transcripts were tested in anin vitro splicing assay. We found that splicing is strongly inhibited if all uridines (U) in the transcript were substituted for BrU. Splicing was restored to some extent if 50% of the Us were replaced by BrU. The splicing efficiency returned to an almost normal level if only I out of every 10 Us was substituted for BrU. This demonstrates that only a pre-mRNA containing a small amount of BrU can be spliced normallyin vitro. Furthermore, these results strongly suggest that some Us in the adenoviral transcript, probably those at the splice sites, cannot be replaced by BrU and are therefore critical in the splicing reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997156
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    Paper (German National Licenses)
    Fulltext
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