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  • AbbreviationsNAA: Naphthaleneacetic acid  (1)
  • Helianthus (embryo-sac isolation)  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Observations on enzymatically isolated, living and fixed embryo sacs in several angiosperm species (1985)
    Springer
    Planta 165 (1985), S. 225-231 
    Details
    ISSN: 1432-2048
    Keywords: Antirrhinum (embryo-sac isolation) ; Embryo sac (isolated) ; Helianthus (embryo-sac isolation) ; Nicotiana (embryo-sac isolation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A technique has been developed for isolating embryo sacs (ESs) by enzymatic maceration. Ovules were macerated in a mixture of pectinase, cellulase and, in some cases, snailase and pectolyase Y-23. The ovular tissues were removed and the ESs were isolated in toto. Embryo sacs were isolated from both fixed and fresh ovules of Antirrhinum majus L., Helianthus annuus L. and Nicotiana tabacum L. Fluorochromasia by fluorescein diacetate showed that the ESs isolated from fresh ovules were viable. The method has promise for various histochemical and cell-physiological studies and quite possibly also for in-vitro culture of ESs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395045
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Gene transfer into isolated and cultured tobacco zygotes by a specially designed device for electroporation (2000)
    Springer
    Plant cell reports 19 (2000), S. 1184-1187 
    Details
    ISSN: 1432-203X
    Keywords: Keywords Zygote ; In vitro culture ; Electroporation ; Transformation ; Tobacco ; AbbreviationsNAA: Naphthaleneacetic acid ; ¶BA: 6-Benzyladenine ; GUS: β-Glucuronidase ; ¶GFP: Green fluorescent protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have established a technique for isolating, culturing and transforming tobacco zygotes. Zygotes were isolated by microdissection or enzymatic maceration from fertilized embryo sacs. Viable zygotes cocultured with mesophyll protoplasts underwent first division after 3 days of culture. Zygotes isolated by microdissection underwent a higher frequency of first division (61.2%) than those isolated by enzymatic maceration (30.5%). Globular embryos were formed only from microdissected zygotes, at a frequency of 8.7% after 1–2 weeks in culture. An efficient millicell device for the electroporation of DNA into zygotes was established. The electroporated zygotes divided in vitro at a frequency of 54.6% and developed into proembryos. Introduced GFP gene constructs showed transient expression in about 2.6% of the electroporated tobacco zygotes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000249
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    Paper (German National Licenses)
    Fulltext
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