ISSN:
1433-2981
Keywords:
Key words:Antimicrobial peptides – Bactenecins, Bovine – Immunofluorescence assay – Monoclonal anitbody – Tissue expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract: Various antimicrobial cationic peptides have been isolated from the granules of bovine neutrophils. One of these peptides is only 12 amino acids in length and is called a dodecapeptide bactenecin. A monoclonal antibody to the synthetic bovine dodecapeptide bactenecin was produced, characterised and used in an immunofluorescent assay to assess the peptide’s expression. When adult bovine peripheral blood and both adult and fetal bone marrow, tracheal lining, liver, spleen and intestinal tissues were examined, the peptide was found only within neutrophils and cells within the bone marrow. These findings both confirm and supplement another report in the literature (Storici et al. 1992), however this is the first report of a bovine antimicrobial peptide detected within the bone marrow of fetal mammals. Our results also suggest the use of immunofluorescence assays to be especially well suited for the quick screening of antimicrobial peptide tissue expression.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s005800050022
Permalink
