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  • 1
    Electronic Resource
    Electronic Resource
    Cloning, expression and characterization of a gene which encodes a topoisomerase I with positive supercoiling activity in pea (1998)
    Springer
    Plant molecular biology 37 (1998), S. 773-784 
    Details
    ISSN: 1573-5028
    Keywords: heterologous expression ; introduction of positive supercoils in DNA ; Pisum sativum ; RACE-PCR ; relaxation of DNA supercoils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated and sequenced the full length cDNA for topoisomerase I. Using degenerate primers, based on the conserved amino acid sequences of five eukaryotic topoisomerase I, a 386 bp fragment was PCR amplified using pea cDNA as template. This fragment was used as a probe to screen a pea cDNA library. Two partial cDNA clones were isolated which were truncated at the 5′ end. RACE-PCR was employed to isolate the remaining portion of the gene. The total size of the gene was 3055 bp with an open reading frame of 2676 bp. The deduced structure of pea topoisomerase I contain 892 amino acids with a calculated molecular weight of 100 kDa and an estimated pI of 9.3. A comparison of the deduced amino acid sequences of the pea topo I with the other eukaryotic topoisomerases clearly suggested that they are all related. Pea topoisomerase I has been overexpressed in E. coli system and the recombinant topoisomerase purified to homogeneity. The purified protein relaxes both positive and negative supercoiled DNA in the absence of divalent cation Mg2+. In the presence of Mg2+ ions the purified enzyme introduces positive supercoils a unique property not reported in any other organism except in archaebacterial topoisomerase I. Polyclonal antibodies were raised against recombinant topoisomerase I and western blotting with sub-cellular fractions indicated the localization of this topoisomerase in pea nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006086311875
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of vesicular arbuscular mycorrhiza and soluble phosphate onAbelmoscus esculentus (L.) Moench (1982)
    Springer
    Plant and soil 64 (1982), S. 209-213 
    Details
    ISSN: 1573-5036
    Keywords: Abelmoscus esculentus ; Ladies finger ; Mycorrhiza ; Phosphate ; VAM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effect of VA mycorrhiza and soluble phosphorus onAbelmoscus esculentus (L.) Moench was studied in a phosphorus deficient sandy loam soil with pH 5.5. The mycorrhizal infection and spore production were reduced by an increase of added soluble phosphorus. Root, shoot and total plant dry weight were significantly greater in mycorrhizal plants than in non-mycorrhizal controls, at all levels of added soluble P. Mycorrhizal dependency was found to decrease with increase in added soluble P. Depression of growth, as compared with growth at 100% was noticed in mycorrhizal plants when 200% of the recommended P was added.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02184252
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  • 3
    Electronic Resource
    Electronic Resource
    Cloning and characterization of a cDNA encoding topoisomerase II in pea and analysis of its expression in relation to cell proliferation (1999)
    Springer
    Plant molecular biology 41 (1999), S. 125-137 
    Details
    ISSN: 1573-5028
    Keywords: cell proliferation ; in vitro transcription and translation ; Pisum sativum ; plant promoter ; primer extension ; RACE-PCR ; transcript analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated and sequenced four overlapping cDNA clones to identify the full-length cDNA for topoisomerase II (PsTopII) from pea. Using degenerate primers, based on the conserved amino acid sequences of other eukaryotic type II topoisomerases, a 680 bp fragment was PCR-amplified with pea cDNA as template. This fragment was used as a probe to screen an oligo-dT-primed pea cDNA library. A partial cDNA clone was isolated that was truncated at the 3′ end. RACE-PCR was employed to isolate the remaining portion of the gene. The total size of PsTopII is 4639 bp with an open reading frame of 4392 bp. The deduced amino acid sequence shows a strong homology to other eukaryotic topoisomerase II (topo II) at the N-terminus end. The topo II transcript was abundant in proliferative tissues. We also show that the level of topo II transcripts could be stimulated by exogenous application of growth factors that induced proliferation in vitro cultures. Light irradiation to etiolated tissue strongly stimulated the expression of topo II. These results suggest that topo II gene expression is up-regulated in response to light and hormones and correlates with cell proliferation. Besides, we have also isolated and analysed the 5′-flanking region of the pea TopII gene. This is first report on the isolation of a putative promoter for topoisomerase II from plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006352820788
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  • 4
    Electronic Resource
    Electronic Resource
    Expression of transformation in cell hybrids. II. Nonsuppression of the transformed phenotype in hybrids between a chemically transformed and nontransformed derivatives of Balb/3T3 (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 97 (1978), S. 147-152 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Hybrid clones derived from a nitrosocarbaryl-transformed Balb/3T3 cell line, Clone H, and a nontransformed cell line TH02 resemble the transformed parent in the clone morphology, higher saturation density, colony formation in medium with reduced serum concentration, growth in agarose and ability to form clones on Balb/3T3 monolayer. Results are discussed in the framework of genetic models which permit or require dominant mutations for the expression of transformed phenotype.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1040970203
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  • 5
    Electronic Resource
    Electronic Resource
    Initial studies on the effects of combined 60 Hz electric and magnetic field exposure on the immune system of nonhuman primates (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 16 (1995), S. 93-102 
    Details
    ISSN: 0197-8462
    Keywords: baboon (Papio cynocephalus) ; interleukin 2 ; mitogen response ; peripheral blood lymphocytes ; T helper/suppressor cell ratio ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In a pilot immunology experiment, peripheral blood samples from six baboons (Papio cynocephalus) housed as a social group were collected during week 5 of preexposure, exposure, and postexposure periods that were each 6 weeks in duration. The subjects were exposed to vertical 6 kV/m and horizontal 50 μT (0.5 G) fields for 12 h per day. Lymphocytes collected during the exposure period displayed statistically significant (P 〈.05) reductions in CD3+ and CD4+ counts, interleukin 2 receptor expression, and proliferative response to pokeweed mitogen. A second experiment was conducted using samples from seven subjects exposed to 30 kV/m and 100 μT (1.0 G) and eight sham-exposed subjects. Statistically significant Period × Group interactions occurred for total white blood cell count and CD4+ to CD8+ ratio, but the pattern of results was not suggestive of an exposure-related effect. Although components of the nonhuman primate immune system appear to be affected by 60 Hz electric and magnetic field exposure in one of two experiments, additional experiments are required to evaluate this possibility. © 1995 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bem.2250160709
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