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  • 1
    Electronic Resource
    Electronic Resource
    Protoplasmic streaming inAcetabularia (1981)
    Springer
    Protoplasma 109 (1981), S. 143-157 
    Details
    ISSN: 1615-6102
    Keywords: Acetabularia ; Microfilaments ; Microtubules ; Protoplasmic streaming
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A compilation of characteristics of the two different systems of intracellular transport inAcetabularia (Koop andKiermayer 1980 a and b) is given. The presence of microfilaments-presumably F-actin-in the cytoplasm ofAcetabularia is demonstrated by electron microscopy. The evidence for an involvement of microtubules in streaming is strengthened by the induction of birefringent vinblastine crystals in the stalk of vegetative cells. Isolated portions of cytoplasm formin vitro more than 100 μm long filopodium-like processes, which are highly birefringent. The processes show intensive immunofluorescent staining with both, anti-actin and anti-tubulin as a primary antibody. A perfusion buffer is presented, which after replacing the vacuolar sap does not lead to a change in cytoplasmic morphology or streaming pattern and velocities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287636
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The flagellar root system in the gamete ofAcetabularia mediterranea (1981)
    Springer
    Protoplasma 109 (1981), S. 257-269 
    Details
    ISSN: 1615-6102
    Keywords: Acetabularia ; Dasycladalean green alga ; Flagellar roots ; Microtubules ; Striated fiber ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Ultrastructural investigation of the flagellar root system ofAcetabularia gametes reveals one type of organization for both male and female gametes. There is a modified cruciate system with four microtubular bands X-2-X-2, with X=4. A prominent distal striated fiber and a small proximal striated fiber connect the flagellar bases. A striated root fiber type I underlies the microtubular root type II, and a short striated root fiber type I underlies the microtubular root type I (terminology ofMelkonian 1980 b). This specific root system has some details in common with theChlamydomonas type, and others with theUlvaphyceae and the siphonalean algaeDerbesia andBryopsis. This might indicate the phylogenetic relationships.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01287446
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Observations on the morphogenetic capacity of “residual nuclei” inAcetabularia (1979)
    Springer
    Protoplasma 101 (1979), S. 363-371 
    Details
    ISSN: 1615-6102
    Keywords: Acetabularia ; Morphogenesis ; Residual nuclei
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Stalks ofAcetabularia mediterranea cells after cyst formation contain “residual nuclei”,e.g., secondary nuclei not used for cyst formation. Residual nuclei may lead to the formation in the stalk of “direct germlings” (Hämmerling 1955), cysts, and—without preceeding cyst formation—of biflagellate swarmers, identical in appearance with gametes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276975
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Protoplasmic streaming in the giant unicellular green algaAcetabularia mediterranea (1980)
    Springer
    Protoplasma 102 (1980), S. 147-166 
    Details
    ISSN: 1615-6102
    Keywords: Acetabularia ; Cell differentiation ; Microcinematography ; Protoplasmic streaming
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplasmic Streaming inAcetabularia mediteranea has been studied by microcinematography in 1. germinating zygotes, 2. germlings before the differentiation of rhizoids and apices, 3. young cells with rhizoids and apices, 4. vegetative cells-several centimeters in length, 5. cells with a maximum sized cap, containing secondary nuclei, and 6. cells after cyst formation. Intracellular transport is found to occur at a network-system of “thin filaments” and at a different system of “headed streaming bands”. At the network of “filaments” chloroplasts are found to move at a velocity of 1–2 Μm/sec. “Headed streaming bands” move along the “filaments” and may lead without interruption from the rhizoid to the apex of the cell andvice versa. The front zone of the “streaming bands” is occupied by a leading cytoplasmic “head”-structure. Small vesicles, polyphosphate granula and secondary nuclei are the predominant moving structures in “headed streaming bands”. The velocity of these particles is found to be 3–11 Μm/sec. The filament system is found during all developmental stages. “Headed streaming bands” are undetectable in germinating zygotes and develop from small cytoplasmic droplets in germlings to broad heavily loaded bands in the huge vegetative cell. Transport of secondary nuclei by “headed streaming bands” is not observed during mitotic divisions and after cyst formation, though moving bands are still present for several weeks after cyst formation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276955
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The life cycle ofAcetabularia (Dasycladales, Chlorophyceae): A compilation of evidence for meiosis in the primary nucleus (1979)
    Springer
    Protoplasma 100 (1979), S. 353-366 
    Details
    ISSN: 1615-6102
    Keywords: Acetabularia ; Chlorophyceae ; Life cycle ; Meiosis ; Nucleus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The life cycle ofAcetabularia is described with special reference to nuclear divisions. Recent arguments, derived from the fields of cytology, genetics and systematics are in favour of the hypothesis, that meiosis occurs during the division of the primary nucleus. This hypothesis is summarized in a schematical representation of the whole life cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279322
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Protoplasmic streaming in the giant unicellular green algaAcetabularia mediterranea (1980)
    Springer
    Protoplasma 102 (1980), S. 295-306 
    Details
    ISSN: 1615-6102
    Keywords: Acetabularia ; Inhibitors ; Microcinematography ; Microfilaments ; Microtubules ; Protoplasmic streaming
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sensitivity of the dual intracellular transport system inAcetabularia mediterranea (Koop andKiermayer 1979 a) to cytochalasin B (CB, 10−5 mol), chlorisopropyl-N-phenylcarbamate (CIPC, 2.10−4 mol), and amiprophosmethyl (APM, 3.10−5 mol) has been studied by microcinematography. Vegetative cells before cap formation, 2–3 cm in length, and cells with a maximum sized cap, containing secondary nuclei, were used for the experiments. All intracellular movements ceased under the influence of CB, while in contrast to “headed streaming bands” and to the migration of the secondary nuclei, the movement of chloroplasts at “thin filaments” was found to be insensitive to Col, CIPC, and APM. All inhibitory effects of the drugs on protoplasmic streaming were completely reversible within a time of less than 10–20 minutes recovery from the drugs. The results suggest an involvement of microfilaments in all intracellular movements while, in addition, microtubules seem to be connected with the movement on “headed streaming bands”, including the migration of secondary nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279593
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    Paper (German National Licenses)
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