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  • 1
    Electronic Resource
    Electronic Resource
    Biochemical evidence for multiple forms of acetohydroxy acid synthase in Spirulina platensis (1991)
    Springer
    Archives of microbiology 155 (1991), S. 298-302 
    Details
    ISSN: 1432-072X
    Keywords: Acetohydroxy acid synthase ; Acetolactate synthase ; Isozymes ; Isoleucine-leucine-valine biosynthesis ; Spirulina ; Cyanobacteria ; Flavin adenine dinucleotide requirement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two isoforms of acetohydroxy acid synthase (AHAS), the first enzyme of the branched-chain amino acids biosynthetic pathway, were detected in cell-free extracts of the cyanobacterium Spirulina platensis and separated both by ion-exchange chromatography and by hydrophobic interaction. Several biochemical properties of the two putative isozymes were analysed and it was found that they differ for pH optimum, FAD requirement for both activity and stability, and for heat lability. The results were partially confirmed with the characterization of the enzyme extracted from a recombinant Escherichia coli strain transformed with one subcloned S. platensis coli strain transformed with one subcloned S. platensis AHAS gene. The approximate molecular mass of both AHAS activities, estimated by gel filtration, indicates that they are distinct isozymes and not different oligomeric species or aggregates of identical subunits.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252216
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Molecular cloning and expression of Spirulina platensis acetohydroxy acid synthase genes in Escherichia coli (1991)
    Springer
    Archives of microbiology 155 (1991), S. 360-365 
    Details
    ISSN: 1432-072X
    Keywords: Acetohydroxy acid synthase ; Ilv genes ; Cyanobacteria ; Isoleucine ; Leucine ; Valine biosynthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The coding sequence for Spirulina platensis acetohydroxy acid synthase (AHAS, EC 4.1.3.18) is shown to be contained within a 4.2 Kb ClaI fragment (ilvX) that has been cloned from a recombinant lambda library. This fragment was able to complement a suitable mutant of Escherichia coli when inserted into the ClaI site of plasmid pAT153 in either orientation, demonstrating that transcription of ilvX originated within the cloned fragment. The probe used for hybridization experiments was the corresponding gene from Anabaena sp. PCC7120. The same probe allowed us to identify a second putative gene encoding AHAS in the S. platensis genomic library.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00243456
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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