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  • 1
    Electronic Resource
    Electronic Resource
    The development of sulfonylurea herbicide-resistant birdsfoot trefoil (Lotus corniculatus) plants from in vitro selection (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 480-488 
    Details
    ISSN: 1432-2242
    Keywords: Lotus corniculatus ; Herbicide resistance ; Sulfonylurea herbicide ; In vitro selection ; Acetolactate synthase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Herbicide-resistant lines of birdsfoot trefoil (Lotus corniculatus L. cv ‘Leo’) were isolated after sequential selection at the callus, shoot, and whole plant levels to the sulfonylurea (SU) herbicide Harmony {DPX-M6316; 3-[[[(4-methoxy-6methyl-1,3,5, triazine-2-yl) amino] carbonyl] amino] sulfonyl-2-thiophenecarboxylate}. In field and growth chamber tests the Harmony regenerant lines displayed an increased tolerance as compared to control plants from tissue culture and controls grown from seed. Results of evaluation of callus cultures of regenerated mutant lines signify stability of the resistance. Outcrossed seeds collected from field trials, and tested in vitro for herbicide resistance, indicate that the trait is heritable and that resistance may be due to reduced sensitivity of acetolactate synthase to SU inhibition. Genetically stable herbicide-resistant lines of birdsfoot trefoil were successfully isolated using in vitro selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226537
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    In situ hybridization as a rapid means to assess meiotic pairing and detection of alien DNA transfers in interphase cells of wide crosses involving wheat and rye (1991)
    Springer
    Molecular genetics and genomics 225 (1991), S. 33-37 
    Details
    ISSN: 1617-4623
    Keywords: Biotin-labelled DNA ; In situ hybridization ; Meiosis ; Interphase ; Wild germplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The objectives of this study were to determine if biotin-labelled total genomic DNA of rye (Secale cereale L.) could be used to (i) preferentially label rye meiotic chromosomes in triticale and (ii) detect translocation stocks at interphase and/or early prophase by in situ hybridization. Welsh triticale, a wheat-rye segmental amphiploid, and Kavkaz wheat, a wheat-rye translocation were used. The results indicated that labelled chromosomes of rye and unlabelled chromosomes of wheat could be observed throughout all meiotic stages in the triticale. For Kavkaz wheat, the presence of the translocated 1RS chromosome arm of rye was detected at the interphase or very early prophase stage. Rapid assessment of feasibility of gene transfers and detection of alien DNA in somatic cells at the interphase stage by in situ hybridization allows for rapid decision-making and saves time and expense in plant breeding programs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00282639
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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