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  • 1
    Electronic Resource
    Electronic Resource
    ATP formation coupled to caffeate reduction by H2 in Acetobacterium woodii NZva16 (1988)
    Springer
    Archives of microbiology 150 (1988), S. 447-451 
    Details
    ISSN: 1432-072X
    Keywords: Acetobacterium woodii ; Caffeate reduction ; ATP formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have addressed the question, whether the reduction of caffeate in Acetobacterium woodii strain NZva16 is coupled to ATP synthesis by electron transport phosphorylation. The following results were obtained: 1. Cultures of A. woodii with H2 and CO2, grew to greater cell densities, when caffeate was also present. Caffeate was reduced to give hydrocaffeate and less acetate was formed. The cell yield based on the amount of caffeate reduced was approximately 1 g dry cells/mol. 2. Non-growing bacterial suspensions catalyzed the reduction of caffeate by H2. The specific activity (0.2–1.0 μmol · min−1 · mg−1 bacterial protein) was as high as expected for a catabolic reaction. 3. The ATP content of bacteria incubated, with H2 increased from 〈 1 to about 7 μmol per g cellular protein on the addition of caffeate. The ATP yield was calculated as 0.06 mol ATP · mol−1 caffeate from the initial velocity of ATP formation and the activity of caffeate reduction. Valinomycin together with nigericin inhibited ATP formation and caused a 2–3-fold increase of the activity of caffeate reduction. Protonophores were without, effect. 4. Caffeate in the presence of H2 caused the uptake of tetraphenylphosphonium cation by the bacteria. The uptake was abolished by valinomycin plus nigericin, and was considerably enhanced by monensin. Protonophores were without effect, even in the presence of monensin. It is concluded that caffeate reduction by H2 is coupled to ATP formation by electron transport phosphorylation. However, the failure of protonophores to prevent phosphorylation and TPP uptake cannot be explained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00422285
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  • 2
    Electronic Resource
    Electronic Resource
    Oxidation of organic compounds to CO2 with sulfur or thiosulfate as electron acceptor in the anaerobic hyperthermophilic archaea Thermoproteus tenax and Pyrobaculum islandicum proceeds via the citric acid cycle (1994)
    Springer
    Archives of microbiology 162 (1994), S. 286-294 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Thermoproteus tenax ; Pyrobaculum ; islandicum ; Hyperthermophiles ; Archaea ; Acetyl-CoA oxidation ; Citric acid cycle ; Sulfur respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      The oxidation of organic compounds with elemental sulfur or thiosulfate as electron acceptor was studied in the anaerobic hyperthermophilic archaea Thermoproteus tenax and Pyrobaculum islandicum. T. tenax was grown on either glucose or casamino acids and sulfur; P. islandicum on peptone and either elemental sulfur or thiosulfate as electron acceptor. During exponential growth only CO 2 and H2S rather than acetate, alanine, lactate, and succinate were detected as fermentation products of both organisms; the ratio of CO2/H2S formed was 1 : 2 with elemental sulfur and 1 : 1 with thiosulfate as electron acceptor. Cell extracts of T. tenax and P. islandicum contained all enzymes of the citric acid cycle in catabolic activities: citrate synthase, aconitase, isocitrate dehydrogenase (NADP+ -reducing), oxoglutarate : benzylviologen oxidoreductase, succinyl-CoA synthetase, succinate dehydrogenase, fumarase and malate dehydrogenase (NAD+-reducing). Carbon monoxide dehydrogenase activity was not detected. We conclude that in T. tenax and P. islandicum organic compounds are completely oxidized to CO2 with sulfur or thiosulfate as electron acceptor and that acetyl-CoA oxidation to CO2 proceeds via the citric acid cycle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00301853
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    Paper (German National Licenses)
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