ISSN:
1432-0878
Keywords:
Melanotrophs
;
Immuno-gold technique
;
Secretory vesicles
;
γ 3-MSH
;
α-MSH
;
Acetylation
;
Rabbit
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Melanotrophic cells of the pars intermedia of the rabbit were studied at the electron-microscopic level by means of the immuno-gold technique with the use of antisera against γ 3-MSH and α-MSH. Both antibodies labelled all secretory vesicles stored in the peripheral cytoplasm, but secretory vesicles and intrasaccular condensations in the Golgi area reacting for γ 3-MSH were not labelled with the antibody against α-MSH. Since this antibody appears to recognise only the acetylated form of α-MSH, the present observations suggest that acetylation occurs at a stage subsequent to the Golgi packaging, during maturation of the secretory vesicles. Thus, the morphological evidence supports biochemical data in favour of intragranular processing of opiomelanocortin in melanotrophic cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00216197
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