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  • 1
    Electronic Resource
    Electronic Resource
    Intracellular localization and function of DNA repair methyltransferase in human cells
    Amsterdam : Elsevier
    Mutation Research/DNA Repair 315 (1994), S. 199-212 
    Details
    ISSN: 0921-8777
    Keywords: Anti-MGMT antibody ; DNA repair ; In situ immunostaining ; Intracellular localisation ; O^6-Methylguanine-DNA methyltransferase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0921-8777(94)90032-9
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Phospholipid analysis of alveolar macrophages and bronchoalveolar lavage fluid following bleomycin administration to rabbits (1994)
    Springer
    Lung 172 (1994), S. 91-102 
    Details
    ISSN: 1432-1750
    Keywords: Alveolar macrophag(s) ; Pulmonary surfactant ; Phospholipid(s) ; Bleomycin-Pulmonary injury
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Changes in the lipid metabolism of the lung during pulmonary injury were investigated by quantitative and qualitative analysis of phospholipids in pulmonary surfactant and alveolar macrophages (AM) obtained from rabbits that had been given a single transtracheal injection of bleomycin hydrochloride (BLM) 0, 7, 14, 21, and 28 days previously. BLM treatment increased the phospholipid content of both bronchoalveolar lavage (BAL) supernatant fluids and BAL cells. Furthermore, the proportion of phosphatidylcholine (PC) showed an increase in BAL cells during the development of pulmonary injury, and BLM treatment appeared to cause transformation of AM to foamy AM. Lipid analyses of the foamy AM revealed that their phospholipid content was increased, and that the percentage of PC with palmitic acid was elevated. Thus it appears that accumulation of phospholipids derived from pulmonary surfactant contributes to the increase in phospholipids and PC in BAL cells. These findings indicate that BLM treatment produces an alteration in the amount and composition of AM phospholipids, and also in BAL supernatant fluids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00185080
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Characterization of the acetylcholine-sensitive muscarinic K+ channel in isolated feline atrial and ventricular myocytes (1995)
    Springer
    The journal of membrane biology 145 (1995), S. 143-150 
    Details
    ISSN: 1432-1424
    Keywords: Patch clamp technique ; Acetylcholine ; Muscarinic K+ channel ; GTP — G protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract M2-cholinergic receptor activation by acetylcholine (ACh) is known to cause a negative inotropic and chronotropic action in atrial tissues. This effect is still controversial in ventricular tissues. The ACh-sensitive muscarinic K+ channel (I K(ACh)) activity was characterized in isolated feline atrial and ventricular myocytes using the patch-clamp technique. Bath application of ACh (1 μm) caused shortening of action potential duration without prior stimulation with catecholamines in atrial and ventricular myocytes. Resting membrane potential was slightly hyperpolarized in both tissues. These effects of ACh were greater in atrium than in ventricle. ACh increased whole-cell membrane current in atrial and ventricular myocytes. The current-voltage (I-V) relationship of the ACh-induced current in ventricle exhibited inward-rectification whose slope conductance was smaller than that in atrium. In single channel recording from cell-attached patches, I K(ACh) activity was observed when ACh was induced in the pipette solution in both tissues. The channel exhibited a slope conductance of 47 ±1 pS (mean ± sd, n=14) in atrium and 47 ±2 pS (n= 10) in ventricle (not different statistically; ns). The open times were distributed according to a single exponential function with mean open lifetime of 2.0±0.3 msec (n= 14) in atrium and 1.9±0.3 msec (n=10) in ventricle (ns); these conductance and kinetic properties were similar between the two tissues. However, the relationship between the concentration of ACh and single channel activity showed a higher sensitivity to ACh in atrium (IC 50 =0.03 μm) than in ventricle (IC 50 =0.15 μm). In excised inside-out patches, ventricular I K(ACh) required higher concentrations of GTP to activate the channel compared to atrial channels. These results suggest a reduced I K(ACh) channel sensitivity to M2-cholinergic receptor-linked G protein (Gi) in ventricle compared to atrium in feline heart.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237372
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    Paper (German National Licenses)
    Fulltext
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