Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Einflüsse der Denervation von Muskelendplatten auf die Lokalisation der Acetylcholinesterase und die Bindung von Decamethonium (1970)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 266 (1970), S. 101-112 
    Details
    ISSN: 1432-1912
    Keywords: Muscle Endplate ; Denervation ; Acetylcholinesterase ; Decamethonium ; Oholinergic Receptor ; Muskelendplatte ; Denervation ; Acetylcholinesterase ; Decamethonium ; cholinergischer Receptor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using contact-autoradiography of the whole mouse diaphragm, the distribution of14C-labelled decamethonium during the degeneration of the phrenic nerve was investigated, and compared with cholinesterase staining. Four days after denervation uniform radioactivity is found over the whole muscle which is independent of the duration of the denervation, whereas in the control (right half of the diaphragm with intact phrenic nerve) the radioactivity remains concentrated in the endplate area. The distribution of the radioactivity is independent of the cholinesterase concentration. Quantitative densitometer measurements give a higher molecule count per endplate for decamethonium than for curare, which corresponds with the diffuse distribution of the cholinergic receptors in the endplate region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00997644
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effect of p-nitrophenyl diazonium fluoroborate on cholinergic mechanisms (1976)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 294 (1976), S. 61-68 
    Details
    ISSN: 1432-1912
    Keywords: p-Nitrophenyl diazonium fluoroborate ; Cholinergic neurons ; Acetylcholine receptor ; Acetylcholinesterase ; Affinity labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Electrophysiological experiments were done to investigate the effect of p-nitrophenyl diazonium fluoroborate (p-NPD) on motor endplates of the frog's m. cutaneus pectoris. The compound has no direct depolarizing effect on the postsynaptic membrane and stabilizes it irreversibly when added to the bath. Longtime iontophoretical applications of p-NPD produce a biphasic effect: initially a potentiation of the depolarizations due to acetylcholine (ACh) (both iontophoretically applied and presynaptically liberated), and subsequently an inhibition of the response to ACh. When the acetylcholinesterase (AChE) is inactivated previously, only the inhibiting effect of the compound is demonstrable. The association constant of p-NPD to purified AChE and to membrane fragments of electroplax was determined by biochemical methods. The compound's affinity to the AChE was found to be about 20 times greater than to the acetylcholine receptor (AChR). Iontophoretical application of p-NPD to cholinergic neurons in the hippocampal cortex of the cat also produced the characteristic biphasic effect on ACh-induced activity of these investigated neurons. The results suggest that the biphasic effect depends on the capacity of p-NPD to combine with both the AChE and the AChR. The AChE is first inhibited with low concentrations thereby potentiating the ACh response. At higher concentrations the AChR's are progressively inhibited too, thereby diminishing the excitability of the postsynaptic membrane up to a complete block.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692785
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...