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  • Acetylcyanamide  (1)
  • Cannabinoide, Enzymimmunoassay  (1)
  • Cytochrome c  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Bacterial genes and proteins involved in the biogenesis of c-type cytochromes and terminal oxidases
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Bioenergetics 1187 (1994), S. 260-263 
    Details
    ISSN: 0005-2728
    Keywords: Assembly ; Cytochrome c ; Heme synthesis ; Heme/copper oxidase ; Membrane transport ; Periplasm
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2728(94)90123-6
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Urinary excretion of acetylcyanamide in rat and human after oral and dermal application of hydrogen cyanamide (H2NCN) (1991)
    Springer
    Archives of toxicology 65 (1991), S. 268-272 
    Details
    ISSN: 1432-0738
    Keywords: Hydrogen cyanamide ; Rat ; Human ; Metabolism ; Urinary excretion ; Acetylcyanamide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The main urinary metabolite of hydrogen cyanamide (syn.: cyanamide) in rat and man is acetylcyanamide (syn.: N-acetylcyanamide). An analytical method was developed to determine acetylcyanamide in the urine with a limit of quantification of 〈10 μg/l (mean recovery 96.1 % using spikes of 20 μg/l; relative standard deviation 〈4%). This methodology is based upon ion chromatography using column-switch techniques and UV detection. It could be demonstrated that in rats an average of 45.6% of oral applied cyanamide (10 mg/kg) was excreted in the urine as acetylcyanamide. In male human volunteers a mean of 40% of oral administered cyanamide (mean dose 0.25 mg/kg body weight) was excreted via the urine as acetylcyanamide. The same group of volunteers participated in a skin absorption study with dermal application of the above cyanamide dose onto a skin surface area of 32 cm2. Within an application period of 6 h an average cyanamide quantity of 2.3 mg was available for skin absorption. A mean portion of 7.7% of this quantity was found as acetylcyanamide in the urine of the participants. Findings from literature state that cyanamide is metabolized in vitro to cyanide. According to examinations performed in vivo, however, such a metabolic pathway seems to be irrelevant for man. In comparison with the control values there was no significant increase of both the cyanide concentrations in the blood and the thiocyanate concentrations in the urine of the above volunteers after the described oral cyanamide administration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968960
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Zum enzymatisch-immunochemischen Nachweis des Haschischkonsums und seiner dünnschichtchromatographischen Absicherung (1985)
    Springer
    International journal of legal medicine 94 (1985), S. 219-225 
    Details
    ISSN: 1437-1596
    Keywords: Cannabinoids, enzyme immunoassay ; THC-metabolite, detection in urine and blood ; Cannabinoide, Enzymimmunoassay ; THC-Metabolit, Nachweis in Urin und Blut
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Mit Hilfe des Enzymimmunoassay (EMIT-dau-Syva) lassen sich Cannabinoide in Blut und Urin nach inhalativer (10 mg THC) oder oraler (30 mg THC) Aufnahme nachweisen. Die Kurvenverläufe werden mitgeteilt. Im Urin waren erhöhte Spiegel bis zu einer Woche nachweisbar. Eine Absicherung der EMIT-Befunde ist mittels Dünnschichtchromatographie nach absorptiver Anreicherung an einer C18-Extraktionssäule möglich. Nachweisgrenze ist 10 ng THC-Carbonsäure/ml bei Einsatz von 10 ml Urin.
    Notes: Summary The EMIT cannabinoid assay was used for screening blood and urine after smoking tetrahydrocannabinol (THC; 10 mg) or ingestion of THC (30 mg). Cannabinoid levels in urine remain detectable up to 1 week. Confirmation was done by adsorption of the THC carboxylic acid onto a C18 extraction column and elution with acetone and TLC. The method is simple and sensitive and is applicable with common laboratory equipment. The detection limit is 10 ng/ml, using 10 ml urine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00204086
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    Paper (German National Licenses)
    Fulltext
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