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  • 1
    Electronic Resource
    Electronic Resource
    Extraction of ribosomal RNA from soil for detection of Frankia with oligonucleotide probes (1990)
    Springer
    Archives of microbiology 154 (1990), S. 329-335 
    Details
    ISSN: 1432-072X
    Keywords: Detection ; Extraction ; Frankia ; Oligonucleotides ; Probes ; Ribosomal RNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequences of 16S rRNA of the nitrogen-fixing Frankia strain Ag45/Mut15 and the ineffective Frankia strain AgB1.9 were used to design a genus-specific oligonucleotide probe. Hybridization experiments of this Frankia probe and a second probe, specific for Nif+-Frankia strains only, were used to detect Frankia specific target sequences in RNA isolations from soil. A method is described for direct isolation of RNA from a loamy soil and a peat. Yields of about 10 ng RNA/g wet soil are obtained without detectable contamination with humic acids. Isolation of RNA after initial extraction of bacteria from soil resulted in significantly lower RNA yields, compared to the direct isolation procedure. Hybridization with both probes against rRNA isolations from Frankia-containing soil could detect target sequences within RNA isolations from 1 g wet soil with an estimated detection limit of 104 cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276527
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Uptake and evolution of H2 and reduction of C2H2 by root nodules and nodule homogenates ofAlnus glutinosa (1979)
    Springer
    Plant and soil 52 (1979), S. 571-578 
    Details
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Actinomycetous symbiosis ; Alnus glutinosa ; Hydrogenase Hydrogen evolution ; Hydrogen uptake ; Nitrogen fixation ; Respiration ; Root nodules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the growing season no net H2 evolution is detected when root nodules ofAlnus glutinosa are incubated in air or in argon containing 20% O2. Due to the hydrogenase activity, N2-fixing root nodules consume added H2 at a rate of about 1.4 μmoles H2.g fresh nodule−1.h−1. The uptake of H2 is only found in summer. At the end of the season, in autumn, nodules evolve significant quantities of H2 although the nodules still continue to fix nitrogen. In-vitro studies with fractionated homogenates of summer-harvested nodules show that the recovery of the hydrogenase is high when using methylene-blue or phenazine metasulfate as electron acceptors. No hydrogenase activity is detected in homogenates of autumn-harvested nodules. The hydrogenase is localised in the microsymbiont.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02277953
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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