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  • Adaptation  (1)
  • Anaerobe  (1)
  • Archaea  (1)
  • CO2 fixation  (1)
  • Conformational energy calculations  (1)
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Material
Years
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Dynamics of the aromatic amino acid residues in the globular conformation of the basic pancreatic trypsin inhibitor (BPTI) (1976)
    Springer
    European biophysics journal 2 (1976), S. 159-180 
    Details
    ISSN: 1432-1017
    Keywords: Conformational energy calculations ; Protein conformation ; Molecular mechanics ; Proteinase inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Summary The molecular conformation of the basic pancreatic trypsin inhibitor (BPTI) is known in considerable detail from both X-ray studies in single crystals and NMR studies in solution. The NMR experiments showed that the aromatic rings of the phenylalanyl and tyrosyl residues can undergo rapid rotational motions about the Cβ-Cγ bond. The present paper describes a model investigation of the mechanistic aspects of these intramolecular rotational motions. From calculations of the conformational energies for molecular species derived from the X-ray structure by rotations of individual aromatic rings, it was apparent that the rotational motions of the aromatics could only be understood in a flexible structure. Flexibility was simulated by allowing the protein to relax to an energetically favorable conformation for each of the different rotation states of the aromatic rings. It was then of particular interest to investigate how the perturbations caused by different rotation states of the aromatic rings were propagated in the protein structure. It was found that the rotation axes Cβ-Cγ were only slightly affected (Δχ 1≲20°). The most sizeable perturbations are caused by through space interactions with nearby atoms, which move away from the ring center and thus release the steric hindrance opposing the rotational motions. The values for the energy barriers obtained from the energy minimization are of the same order of magnitude as those measured by NMR.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00863707
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Thermotoga maritima sp. nov. represents a new genus of unique extremely thermophilic eubacteria growing up to 90°C (1986)
    Springer
    Archives of microbiology 144 (1986), S. 324-333 
    Details
    ISSN: 1432-072X
    Keywords: Evolution ; Eubacteria ; Thermophile ; Anaerobe ; Thermotoga maritima
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel type of bacterium has been isolated from various geothermally heated locales on the sea floor. The organisms are strictly anaerobic, rod-shaped, fermentative, extremely thermophilic and grow between 55 and 90°C with an optimum of around 80°C. Cells show a unique sheath-like structure and monotrichous flagellation. By 16S rRNA sequencing they clearly belong to the eubacteria, although no close relationship to any known group could be detected. The majority of their lipids appear to be unique in structure among the eubacteria. Isolate MSB8 is described as Thermotoga maritima, representing the new genus Thermotoga.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409880
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Methanopyrus kandleri, gen. and sp. nov. represents a novel group of hyperthermophilic methanogens, growing at 110°C (1991)
    Springer
    Archives of microbiology 156 (1991), S. 239-247 
    Details
    ISSN: 1432-072X
    Keywords: Methanopyrus ; Methanogens ; Archaea ; Hyperthermophilic ; Marine ; Vents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel group of hyperthermophilic rod-shaped motile methanogens was isolated from a hydrothermally heated deep sea sediment (Guaymas Basin, Gulf of California) and from a shallow marine hydrothermal system (Kolbeinsey ridge, Iceland). The grew between 84 and 110°C (opt: 98°C) and from 0.2% to 4% NaCl (opt. 2%) and pH 5.5 to 7 (opt: 6.5). The isolates were obligate chemolithoautotrophes using H2/CO2 as energy and carbon sources. In the presence of sulfur, H2S was formed and cells tended to lyse. The cell wall consisted of a new type of pseudomurein containing ornithin in addition to lysine and no N-acetylglucosamine. The pseudomurein layer was covered by a detergent-sensitive protein surface layer. The core lipid consisted exclusively of phytanyl diether. The GC content of the DNA was 60 mol%. By 16S rRNA comparisons the new organisms were not related to any of the three methanogenic lineages. Based on the physiological and molecular properties of the new isolates, we describe here a new genus, which we name Methanopyrus (the “methane fire”). The type species is Methanopyrus kandleri (type strain: AV19; DSM 6324).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262992
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Enzymes of the reductive citric acid cycle in the autotrophic eubacterium Aquifex pyrophilus and in the archaebacterium Thermoproteus neutrophilus (1993)
    Springer
    Archives of microbiology 160 (1993), S. 306-311 
    Details
    ISSN: 1432-072X
    Keywords: Autotroph ; Archaebacteria ; Aquifex ; Hydrogenobacter ; Thermoproteus ; CO2 fixation ; Reductive citric acid cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The autotrophic carbon fixation pathway was studied in the thermophilic hydrogen oxidizing eubacterium Aquifex pyrophilus and in the thermophilic sulfur reducing archaebacterium Thermoproteus neutrophilus. Neither organism contained ribulose-1,5-bisphosphate carboxylase activity suggesting that the Calvin cycle is not operating. Rather, all enzymes of the reductive citric acid cycle were found in A. pyrophilus. In T. neutrophilus ATP citrate lyase activity was detected which has not been achieved so far; this finding corroborates earlier work suggesting the presence of the reductive citric acid cycle in this archaebacterium. The reductive citric acid cycle for autotrophic CO2 fixation now has been documented in the eubacterial branches of the proteobacteria, in green sulfur bacteria, and in the thermophilic Knallgas bacteria as well as in the branch of the sulfur dependent archaebacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292082
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  • 5
    Electronic Resource
    Electronic Resource
    Brain morphology and turbidity preference in Notropis and related genera (Cyprinidae, Teleostei) (1992)
    Springer
    Environmental biology of fishes 33 (1992), S. 153-165 
    Details
    ISSN: 1573-5133
    Keywords: Neuroanatomy ; Ecology ; Vision ; Olfaction ; Gustation ; Plasticity ; Adaptation ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis The size of seven neural structures was compared in 51 species of Notropis, Pteronotropis, Cyprinella, Luxilus, Lythrurus, and Hybopsis, and related to the turbidity of the species& habitat. This last parameter was assessed for each species by personal communication with 42 ichthyologists. To control for size differences among species, all analyses were performed on the residuals from a regression of each character on standard length. Principal components analysis (PCA) of the residuals produced four significant PC-axes that together explained 65% of the total variation represented in the original variables. The size of brain structures concerned with vision, olfaction, and gustation was correlated with habitat turbidity. Two-way Analyses of Covariance (ANCOVAs) revealed significant differences between species in the size of all structures. Sexual dimorphism was found in the size of the olfactory bulb and the cerebellum, and significant two-way interactions (species vs. sex) were detected for the telencephalon, optic lobes, cerebellum, vagal lobe, and the eye. Cluster analysis indicated that neither similar turbidity preference nor shared phylogeny is alone sufficient to explain the observed differences in brain morphology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00002561
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    Paper (German National Licenses)
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