ISSN:
0887-3585
Keywords:
X-ray crystallography
;
POU-domain
;
transcription factor
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Medicine
Notes:
The POU domain, representing an approximately 150 amino acid conserved region, serves as the DNA-recognition domain for a large number of eukaryotic transcription factors. Bipartite in nature, the POU domain is comprised of a N-terminal POU-specific domain connected by a linker of variable length to a C-terminal homeodomain. We report here co-crystals of pituitary-specific factor Pit-1 POU domain bound as a dimer to a 28 bp DNA fragment. The crystals diffract to at least 2.3 Å in resolution and belong to space group P1 with unit cell dimensions of a = 42.5 Å, b = 50.1 Å, c = 55.8 Å, α = 76.7°, β = 79.3°, and γ = 67.2°.
Additional Material:
1 Tab.
Type of Medium:
Electronic Resource
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