Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Adherence reactions  (1)
  • T cells  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Preferential binding of monocytes and Leu 2+T lymphocytes to interferon-gamma treated cultured skin endothelial cells and keratinocytes (1988)
    Springer
    Archives of dermatological research 280 (1988), S. 235-245 
    Details
    ISSN: 1432-069X
    Keywords: Gamma interferon ; Keratinocytes ; Endothelial cells ; Adherence reactions ; T-cell subsets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Recombinant gamma interferon (r-IFN-γ) increases the adherence of peripheral blood mononuclear leukocytes (PBMLs) to cultured keratinocytes and cutaneous microvascular endothelial cells (MECs). To determine which specific type of PBMLs bound to these r-IFN-γ treated cells, we performed immunophenotyping on the adherent PBMLs. The adherent PBMLs were detached from the r-IFN-γ treated keratinocytes and MECs by adding EDTA, and collected by cytocentrifugation, followed by immunocytochemical staining using a panel of monoclonal antibodies. Our results reveal that the relative adherent population of PBMLs was composed of approximately 60%–70% monocytes and 18%–24% Leu 2+T lymphocytes (T-cytotoxic/suppressor) which preferentially bound to r-IFN-γ treated keratinocytes and MECs. There was some lesser binding by Leu 3+ lymphocytes (T-helper/inducer); approximately 8%, and no binding of B lymphocytes. Since r-IFN-γ also induced HLA-DR expression in keratinocytes and MECs, these in vitro data suggest that r-IFN-γ may play an important role in the immunobiology of diverse skin diseases such as graft vs host disease, lichen planus, and other inflammatory dermatoses, because the keratinocytes express HLA-DR and the predominant T-cell subset in the epidermis is Leu 2+ (over the Leu 3+T cell) in all of these conditions. These results represent a direct attempt to explain in situ immunophenotypic mononuclear leukocyte subset distribution patterns by using r-IFN-γ and purified cultured cells such as keratinocytes and MECs. We propose that IFN-γ, by both increasing the adherence of PBMLs, and promoting selective binding of monocytes and Leu 2+T lymphocytes to both keratinocytes and MECs, may be important in regulating PBML localization and recirculation in the skin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00513963
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Effect of neuropeptides present in skin on the proliferation of human peripheral blood mononuclear cells and T cells (1988)
    Springer
    Archives of dermatological research 280 (1988), S. 279-281 
    Details
    ISSN: 1432-069X
    Keywords: Substance P ; Neuropeptides ; PBML ; T cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The release of neuropeptides, such as substance P (SP) and somatostatin (SOM), from primary sensory nerve fibers has been implicated in the modulation of local immune responses in surface tissues, such as the skin, the pulmonary airways, and the gastrointestinal mucosa. We have investigated the influence of six neuropeptides substance P (SP), somatostatin (SOM), substance K (SK), vasoactive intestinal peptide (VIP), bombesin (BOM), and adrenocorticotropic hormone (ACTH) on the proliferation of resting and partially stimulated human peripheral blood mononuclear leukocytes (PBMLs) and T lymphocytes. Neuropeptides in concentrations from 10-7 to 10-12 M were added to either resting or partially stimulated cells [interleukin-2 (IL-2), concanavalin A (Con A), and phytohemagglutinin (PHA)]. Cellular proliferation was assessed by incorporation of 3H-thymidine after 72h. With the exception of SP, no significant effect of any of these neuropeptides on 3H-thymidine incorporation was found. In resting cells, 10-9 MSP elicits an 80... maximal increase of 3H-thymidine incorporation, whereas no statistically significant effect on partially stimulated leukocytes was found. These results contradict a previous report on a significant mitogenic effect of SP on partially stimulated T cells. Considering the very minimal effect of SP on resting cells and, particularly, the absence of an effect on partially stimulated cells, we would question a significant modulatory role for SP and the five other neuropeptides in the proliferation of immunocompetent cells in skin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00440600
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...