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  • 1
    Electronic Resource
    Electronic Resource
    The effect of conditioned medium on fetal human liver cells in primary culture (1986)
    Springer
    Research in experimental medicine 186 (1986), S. 463-468 
    Details
    ISSN: 1433-8580
    Keywords: Conditioned medium ; Colony ; Human liver ; Primary ; Albumin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A medium conditioned by rat embryo cultures (RCM) promoted the adhesion of liver cells from human fetuses to plastic dish. Colonies were also formed in primary cultures of the same cells in the presence of RCM. The majority of the colonies formed were composed of large polygonal cells with a few colonies composed of both clear epithelial-like cells and fibroblast-like cells. RCM was superior to the rat embryo feeder layer for promotion of colony formation of cells. A number of colonies were formed from fetal human livers when a conditioned medium from human hepatoma cells (HCM) was used, but most of the colonies formed were composed of fibroblast-like cells. The cells derived from the polygonal cell colonies, which were formed in the presence of RCM, have been passaged four times and they are still growing with albumin-producing capacity. The effect of RCM was reduced by various physico-chemical treatments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01852200
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation of clones from T24 cells and test of their sensitivity to adriamycin (1988)
    Springer
    Research in experimental medicine 188 (1988), S. 239-246 
    Details
    ISSN: 1433-8580
    Keywords: T24 ; Heterogeneity ; Single cell clone ; Adriamycin ; Chemosensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two single cell clones, CL3 and CL7, were developed from T24 cells. CL3 had abundant cytoplasmic granules, while CL7 did not have as much. CL3 and CL7 exhibited a modal number of chromosomes at 69 and 74, respectively. CL7 had a higher growth rate than CL3, as indicated by in vitro cell growth, plating efficiency, and growth of the tumors produced in nude mice. CL3 was slightly susceptible to adriamycin toxicity as compared to CL7.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01852272
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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