ISSN:
1432-1432
Keywords:
Xenopus laevis
;
Xenopus tropicalis
;
Adult α-globin genes
;
Gene organization
;
Conserved upstream sequences
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary To investigate the evolution of globin genes in the genusXenopus, we have determined the primary structure of the related adult α1- and αII genes ofX. laevis and of the adult α-globin gene ofX. tropicalis, including their 5′-flanking regions. All three genes are comprised of three exons and two introns at homologous positions. The exons are highly conserved and code for 141 amino acids. By contrast, the corresponding introns vary in length and show considerable divergence. Comparison of 900 bp of the 5′-flanking region revealed that theX. tropicalis gene contains a conserved proximal 310-bp promoter sequence, comprised of the canonical TATA and CCAAT motifs at homologous positions, and five conserved elements in the same order and at similar positions as previously shown for the corresponding genes ofX. laevis. We therefore conclude that these conserved upstream elements may represent regulatory sequences for cell-specific regulation of the adultXenopus globin genes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02143498
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