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  • Adult human  (1)
  • Bulk isolation  (1)
  • Dystrophic axons  (1)
  • Growth hormone  (1)
  • Herpes simplex virus  (1)
Datenquelle
Materialart
Erscheinungszeitraum
Schlagwörter
  • 1
    Digitale Medien
    Digitale Medien
    Isolation, culture and cell-type identification of adult human pituitary cells (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 205-208 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Human pituitary gland ; Tissue culture ; Density gradients ; Immunocytochemistry ; Growth hormone ; Prolactin ; Adrenocorticotropin ; Follicle-stimulating hormone ; Luteinizing hormone ; Thyrotropin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Pituitary cells were isolated from adult human pituitary glands obtained at autopsy 4–12 h postmortem by enzyme treatment (collagenase and dispase) and by Percoll density gradients. Cells thus isolated were maintained in culture for more than 6 months. By immunoperoxidase staining methods using rabbit sera monospecific against various pituitary hormones, a large number of cultured cells reacted positively. The hormones identified in these cells were growth hormone, prolactin, adrenocorticotropin, follicle-stimulting hormone, luteinizing hormone and thyrotropin. Electron-microscopic examination of cultured cells revealed the presence of secretory granules in cytoplasm characteristic of in vivo human pituitary cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00690196
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Morphological alterations of Purkinje cell axons and presynaptic terminals in organotypic cerebellar cultures exposed to ionizing irradiation (1981)
    Springer
    Acta neuropathologica 54 (1981), S. 301-310 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Dystrophic axons ; Ionizing irradiation ; Tissue culture ; Presynaptic terminals
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Ionizing irradiation induced marked morphological alterations in the Purkinje cell axons and axonic terminals in vitro. The myelinated segments demonstrated a very poor development of the myelin sheath. The axoplasm demonstrated various alterations of the mitochondria, and the smooth endoplasmic reticulum and the numerous elongated presynaptic terminals contained large aggregates of tubulovesicular structures and neurofilaments arranged in parallel and reticular array.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00697004
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Culture and cryopreservation of adult human oligodendrocytes and astrocytes (1984)
    Springer
    Acta neuropathologica 64 (1984), S. 172-175 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Adult human ; Oligodendrocyte ; Astrocyte ; Tissue culture ; Cryopreservation ; Bulk isolation ; Immunofluorescence microscopy ; Cell type-specific markers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Human adult oligodendrocytes were bulkisolated from the brains obtained at autopsy by the Percoll density gradient method and cultured in vitro for 2–28 days. Cultured human oligodendrocytes were then scraped from the dishes or coverslips, frozen at −70°C in the presence of 10% dimethyl sulfoxide as a cryoprotectorant, and then stored in liquid nitrogen tank. After the fast thawing, 55%–70% of human oligodendrocytes plus astrocytes were found to survive in the culture. Identification of cell types was established by using antibodies directed against galactocerebroside (oligodendrocyte) and glial fibrillary acidic protein (astrocyte).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00695582
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  • 4
    Digitale Medien
    Digitale Medien
    Herpes simplex virus replication in pheochromocytoma cell line that responds to nerve growth factor (1978)
    Springer
    Acta neuropathologica 44 (1978), S. 9-14 
    Details
    ISSN: 1432-0533
    Schlagwort(e): Herpes simplex virus ; Replication in a pheochromocytoma cell line ; Nerve growth factor ; Morphological changes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Cultured cells of neural origin (PC-12, pheochromocytoma cell line) respond to nerve growth factor (NGF) by extending neurites. These cells whether treated with NGF or not can be infected with herpes simplex virus and produce progeny virus. Viral antigens are detected on the cell surface and fusion of cells to form polykaryocytes takes place. Nucleocapsids are found within the cell nucleus and enveloped virus in present both in the cytoplasm and extracellular space. Virus was not observed within the neurites but alterations in the neurite microtubular structure occurred after infection.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00691633
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