ISSN:
1573-5060
Keywords:
Aegilops markgrafii
;
aneuploids
;
chromosome specific library
;
DOP-PCR
;
microdissection
;
molecular markers
;
Triticum aestivum
;
wheat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5α. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00015717
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