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  • 1
    Electronic Resource
    Electronic Resource
    IS1-mediated mobility of the aerobactin system of pColV-K30 in Escherichia coli (1988)
    Springer
    Molecular genetics and genomics 213 (1988), S. 487-490 
    Details
    ISSN: 1617-4623
    Keywords: pColV ; Aerobactin ; Transposition ; IS1 ; Cointegration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genes determining the high affinity iron transport system mediated by the siderophore aerobactin are flanked in the enterobacterial plasmid pColV-K30 by inverted repeats of IS1 sequences, suggesting that the aerobactin genes are part of a transposon. To study this possibility, the entire region between the two IS1 sequences was cloned as an 18 kb HindIII-BamHI restriction fragment in pUC8 giving plasmid pMO1. A number of derivatives of pMO1, in which aerobactin genes were tagged with a kanamycin resistance gene, were prepared in order to assess the ability of both IS1s to promote the formation of cointegrates with pCJ105, an F derivative devoid of insertion sequences. Mating-out assays indicated that both flanking IS1s were active in cointegrate formation at detectable frequencies. In some cases, the cointegrates could be resolved, the final result being a transposition-like event for the entire aerobactin system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339620
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    A high-affinity iron transport system of Rhizobium meliloti may be required for efficient nitrogen fixation in planta (1991)
    Springer
    Plant and soil 130 (1991), S. 211-217 
    Details
    ISSN: 1573-5036
    Keywords: iron transport ; nitrogen fixation ; nodulation ; Rhizobium ; symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We have analyzed the ability of single site insertion mutants of Rhizobium meliloti 1021 defective in various components of a high-affinity iron transport system to produce nodules, fix nitogen and promote plant growth. Our results indicate that a high-affinity iron transport system may significantly increase the ability of the differentiated form of the bacterium to fix nitrogen and induce an increase in plant growth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00011875
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    Paper (German National Licenses)
    Fulltext
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