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  • 1
    ISSN: 1573-3025
    Keywords: Aerobiology ; Aeromycology ; Spores ; Fungi ; Meteorological factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study of the concentration of fungal spores has been carried out in the atmosphere of Palencia town (NW Spain) during 1992. The volumetric method of filtration has been used. Half of the daily filter sample has been cultivated in Czapecdox-agar or Sabouraud-agar for the identification of fungal colonies, and the other half has been examined by optical microscopy. Several colonies belonging to 26 genera have been identified. Deuteromycetes (54%) and Zigomycetes (28%) are assembled in four genera, and Bacteria and Actynomycetes (18%) in three genera. The greatest concentrations occur forAspergillus (23%),Mucor (25%), followed byPenicillium (16%). The greatest diversity and abundance of fungal spores are found in September–December. The viable colonies are more abundant in Czapedox-agar culture, whereas Bacteria were more frequently found in Sabouraud medium.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-7284
    Keywords: NecrotoxigenicEscherichia coli ; Pathogenicity ; Toxins ; Virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The O:K:H serotypes of 137 necrotoxigenicEscherichia coli (NTEC) producing the cytotoxic necrotizing factor type 1 (CNF1) isolated from human extraintestinal infection were determined. Although NTEC producing CNF1 belonged to 58 different serotypes, only 10 of them accounted for 54% of strains. The most common serotypes, in order of frequency, were: O4:K?:H5, O6:K13:H1, O83:K1:H31, O75:K95:H5, O2:K1:H6, O2:K7:H-, O75:K1:H7, O2:K?:H1, O4:K12:H1 and O22:K13:H1. CNF1 strains of serotypes O2:K7:H- and O4:K12:H1 express P-fimbriae, whereas CNF1 strains of serotypes O2:K?:H1, O2:K1:H6 and O75:K95:H5 possess the adhesin responsible for MRHA type III. Among CNF1 strains of serotype O4:K?:H5 there exist some that express P-fimbriae and others that possess MRHA type III. Lastly, the majority of CNF1 strains of serotypes O6:K13:H1, O22:K13:H1, O75:K1:H7 and O83:K1:H31 do not express P-fimbriae nor the adhesin responsible to MRHA type III. Our results show that extraintestinal infections are caused by a limited number of virulent clones, as suggested by the theory of special pathogenicity.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-7284
    Keywords: Diarrhoea ; E. coli ; Enteropathogens ; Enterotoxins ; Virulence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract One hundred and six enterotoxigenic E. coli (ETEC) isolated from many geographical areas were serotyped and investigated for the presence of colonization factor antigens CFA/I and CFA/II, the expression of mannose-resistant haemagglutination (MRHA) and the levels of surface hydrophobicity. CFA/I was found in 6 (17%) of 36 LT+STa+ strains and in 15 (54%) of 28 STa+ strains; CFA/II was found in 16 (44%) of 36 LT+STa+ strains. None of 42 LT+ strains showed CFA/I or CFA/II. CFA/I was found in ETEC of serotypes O63:K−:H−, O78:K80, O128:K67 and O153:K−:H45, whereas CFA/II was found in serotypes O6:H−, O6:K15:H16 and 06:K?:H40. Of the 69 CFA/I− CFA/II− ETEC strains, 9 (13%) showed MRHA with some of the seven erythrocyte species used and 21 (30%) were hydrophobic. Among the 21 hydrophobic strains CFA-negative we have detected: (i) 6 LT+ strains of serogroup O25 negative for MRHA, (ii) 5 strains O159 (4 LT+ and 1 LT+ STa+) also negative for MRHA, and (iii) 3 STa+ strains of serotype O27:K−:H7 that haemagglutinated calf and sheep erythrocytes when grown on Minca-Is. The 106 ETEC strains belonged to 20 different 0 serogroups. However, 77 (73%) were of one of nine serogroups (O6, O8, O25, O27, O78, O148, O153, O159 and O167). E. coli strains belonging to O6 and O153 groups predominated among ETEC isolated in Spain, O159 strains in the Central African Republic, O25 and O148 strains in Japan, and O15 and O78 strains in India.
    Type of Medium: Electronic Resource
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