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  • 1
    Electronic Resource
    Electronic Resource
    Compaction stasis due to gravitational red cell migration and floatational plasma skimming (1989)
    Springer
    Virchows Archiv 415 (1989), S. 243-251 
    Details
    ISSN: 1432-2307
    Keywords: Erythrocytes ; Velocity profiles ; Aggregation ; Gravitation ; Sedimentation ; Stasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A horizontally aimed microscope was directed at isolated rat mesentery preparations as well as artificial microchannels cast in polyester blocks. They were perfused with aggregating red cell suspensions containing about 7 g/l of bovine fibrinogen at various perfusion pressures. The effects induced by gravitational influences were monitored by measuring the local red cell concentration (video-densitometry) and velocity profiles (IPM-dual slit velocimetry). At low perfusion pressures, sedimentation during maintained flow occurs, leading to a relative red cell slowing compared with plasma. Consequently, a progressive deposition of red cells at the bottom of vessels occurs and finally, blocking of the vessel by aggregated red cells is seen. Thus, the well-known phenomenon of compaction stasis must be attributed not merely to a transmural plasma loss but also to gravitationally induced haemoconcentration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00724911
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The role of aerolysin inAeromonas-associated infections (1987)
    Springer
    Cellular and molecular life sciences 43 (1987), S. 370-371 
    Details
    ISSN: 1420-9071
    Keywords: Aerolysin ; isogenic mutants ; mouse lethality studies ; DNA hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01940412
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Mini-TnhlyAs: a new tool for the construction of secreted fusion proteins (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 266-274 
    Details
    ISSN: 1617-4623
    Keywords: Key words Escherichia coli hemolysin ; Minitransposon ; Secretion of HlyAs-fusion proteins ; TolC protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A simple and efficient procedure for the construction of secreted fusion proteins in Escherichia coli is described that uses a new minitransposon, termed TnhlyAs, carrying the secretion signal (HlyAs) of E. coli hemolysin (HlyA). This transposon permits the generation of random gene fusions encoding proteins that carry the HlyAs at their C-termini. For the construction of model gene fusions we used lacZ, encoding the cytoplasmic β-galactosidase (β-Gal), and phoA, encoding the periplasmic alkaline phosphatase, as target genes. Our data suggest that all β-Gal-HlyAs fusion proteins generated are secreted, albeit with varying efficiencies, by the HlyB/HlyD/TolC hemolysin secretion machinery under Sec-proficient conditions. In contrast, the PhoA-HlyAs fusion proteins are efficiently secreted in a secA mutant strain only under SecA-deficient conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173772
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Mini-TnhlyAs: a new tool for the construction of secreted fusion proteins (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 266-274 
    Details
    ISSN: 1617-4623
    Keywords: Escherichia coli hemolysin ; Minitransposon ; Secretion of HlyAs-fusion proteins ; TolC protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple and efficient procedure for the construction of secreted fusion proteins inEscherichia coli is described that uses a new minitransposon, termed TnhlyAs, carrying the secretion signal (HlyAs) ofE. coli hemolysin (HlyA). This transposon permits the generation of random gene fusions encoding proteins that carry the HlyAs at their C-termini. For the construction of model gene fusions we usedlacZ, encoding the cytoplasmicβ-galactosidase (β-Gal), andphoA, encoding the periplasmic alkaline phosphatase, as target genes. Our data suggest that allβ-Gal-HlyAs fusion proteins generated are secreted, albeit with varying efficiencies, by the HlyB/HlyD/TolC hemolysin secretion machinery under Sec-proficient conditions. In contrast, the PhoA-HlyAs fusion proteins are efficiently secreted in asecA mutant strain only under SecA-deficient conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173772
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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