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  • Aerotaxis  (1)
  • Methionine starvation  (1)
  • Tumble generator  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 96 (1974), S. 83-92 
    ISSN: 1432-072X
    Keywords: Chemotaxis ; Chemoreceptor ; Motility ; Bacillus subtilis ; Aerotaxis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Quantitative measurement of positive chemotaxis inBacillus subtilis was performed by means of adaption of the procedure used in studies withEscherichia coli. The motility ofB. subtilis was optimal in the presence of an exogeneous energy source and a nonionic detergent,e. g. Tween 80 or Brij-36. B. subtilis is chemotactic toward the commonly occurringL-amino acids except arginine, lysine, aspartate and glutamate. No chemotactic response was observed towardD-amino acids. Threshold, optimal response and peak concentration were determined. Chemotaxis toward glutamine was optimal at pH 6-7 and a temperature of 32°C. The maximum response toward a particular attractant was presumably influenced by the aerotactic behavior ofB. subtilis.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 113 (1977), S. 153-158 
    ISSN: 1432-072X
    Keywords: Chemotaxis ; Motility ; Velocity ; Methionine starvation ; Bacillus subtilis 60015
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Constant levels of amino acids enhanced the velocity of Bacillus subtilis 60015 cells about 2-fold and stimulated the response in motility assays. The stimulation of velocity did not occur via the receptors for chemotaxis. Cysteine and methionine, general inhibitors of chemotaxis, both completely inhibited the smooth response in a temporal gradient of attractant. After methionine starvation B. subtilis 60015 showed no measurable response in a temporal gradient of attractant, this in contrast to the effect observed with some other bacteria. Addition of methionine to starved cells restored the response toward attractant. Revertants of B. subtilis 60015 for methionine requirement could not be starved and showed a normal behavior toward temporal gradients of attractant.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 111 (1976), S. 7-11 
    ISSN: 1432-072X
    Keywords: Bacillus subtilis ; Motility ; Chemotaxis ; Chemoreceptor ; Tumble generator ; Proton-motive force
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Changes in the proton-motive force cause a transient change in the motile behavior of Bacillus subtilis cells. Both an increase and a decrease in the proton-motive force cause transient tumbling. Simultaneous decrease of proton-motive force and increase of attractant concentration lessens the response toward the attractant. A simultaneous increase of proton-motive force and increase of attractant concentration prolonges the response toward attractant. A hypothesis explaining the various effects is given.
    Type of Medium: Electronic Resource
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